Journal of Clinical Microbiology, February 1999, p. 296-303, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Instituto de Biotecnología,
Received 4 March 1998/Returned for modification 23 April
1998/Accepted 13 October 1998
Two hundred twenty-four Mycobacterium bovis isolates,
mainly from South American countries, were typed by spoligotyping, and 41 different spoligotypes were identified. A total of 202 M. bovis isolates (90%) were grouped into 19 different clusters.
The largest cluster contained 96 isolates (42.8%) on the basis of the
most frequently observed spoligotype, spoligotype 34. Nineteen M. bovis isolates from humans in Argentina had spoligotypes and
polymorphic GC-rich repetitive sequence (PGRS) types that represented
the most common types found among isolates from cattle. All five
isolates from Uruguay and three of the six isolates from Paraguay had
spoligotypes that were also detected for isolates from Argentina. The
spoligotypes of isolates from Brazil, Costa Rica, and Mexico and of
some of the isolates from Paraguay could not be found in Argentina. A total of 154 M. bovis isolates were selected in order to
compare the discriminative power of spoligotyping and restriction
fragment length polymorphism (RFLP) analysis with direct repeat (DR)
and PGRS probes. By spoligotyping, 31 different types were found, while
AluI-digested DR probe-associated RFLP analysis identified 42 types, and RFLP analysis with the PGRS probe also detected 42 types;
these were partly independent of the DR types. By combining the results
obtained by spoligotyping and by RFLP analysis with the DR and PGRS
probes, 88 different types were obtained. Although the differentiation
of M. bovis by spoligotyping was less discriminatory than
differentiation by RFLP analysis with the DR and PGRS probes, spoligotyping is easier to perform and its results are easier to
interpret. Therefore, for the purpose of typing of M. bovis isolates, spoligotyping could be performed first and the isolates could
be grouped into clusters and then analyzed by RFLP analysis with the DR
and PGRS probes.
*
Corresponding author. Mailing address: Instituto de
Biotecnología, CICV/INTA, PO Box 77 (1708), Morón,
Provincia de Buenos Aires, Argentina. Phone: (54-1) 621-1447. Fax:
(54-1) 4812975. E-mail: mzumarra{at}cicv.inta.gov.ar.
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