This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, K. J. Y.
Right arrow Articles by Kain, K. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, K. J. Y.
Right arrow Articles by Kain, K. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 339-341, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of a Colorimetric PCR-Based Assay To Diagnose Plasmodium falciparum Malaria in Travelers

Kathleen J. Y. Zhong and Kevin C. Kain*

Tropical Disease Unit, Division of Infectious Diseases, Department of Medicine, Toronto Hospital, and the University of Toronto, Toronto, Ontario, Canada

Received 23 June 1998/Returned for modification 20 August 1998/Accepted 2 November 1998

New diagnostic tests are needed to facilitate the diagnosis of Plasmodium falciparum malaria in the returned traveler. We performed a blinded evaluation of a nonisotopic colorimetric PCR-based assay (Digene SHARP Signal System) and compared the results with those obtained by microscopy and nested PCR for the detection of P. falciparum malaria in 150 febrile travelers. By using nested PCR as the reference standard, the colorimetric assay had a sensitivity of 100% and a specificity of 95.4% for the detection of P. falciparum. This PCR-based nonisotopic assay is a rapid, sensitive, and specific method for the detection of falciparum malaria in returned travelers.

* Corresponding author. Mailing address: Tropical Disease Unit, EN G-224, The Toronto Hospital, 200 Elizabeth St., Toronto, Ontario M5G 2C4, Canada. Phone: (416) 340-3535. Fax: (416) 595-5826. E-mail: kkain{at}torhosp.toronto.on.ca.

Journal of Clinical Microbiology, February 1999, p. 339-341, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Perandin, F., Manca, N., Calderaro, A., Piccolo, G., Galati, L., Ricci, L., Medici, M. C., Arcangeletti, M. C., Snounou, G., Dettori, G., Chezzi, C. (2004). Development of a Real-Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis. J. Clin. Microbiol. 42: 1214-1219 [Abstract] [Full Text]  
  • FARCAS, G. A., ZHONG, K. J. Y., LOVEGROVE, F. E., GRAHAM, C. M., KAIN, K. C. (2003). EVALUATION OF THE BINAX NOW(R) ICT TEST VERSUS POLYMERASE CHAIN REACTION AND MICROSCOPY FOR THE DETECTION OF MALARIA IN RETURNED TRAVELERS. Am J Trop Med Hyg 69: 589-592 [Abstract] [Full Text]  
  • Ciach, M., Zong, K., Kain, K. C., Crandall, I. (2003). Reversal of Mefloquine and Quinine Resistance in Plasmodium falciparum with NP30. Antimicrob. Agents Chemother. 47: 2393-2396 [Abstract] [Full Text]  
  • Morata, P., Queipo-Ortuno, M. I., Reguera, J. M., Garcia-Ordonez, M. A., Cardenas, A., Colmenero, J. D. (2003). Development and Evaluation of a PCR-Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Brucellosis. J. Clin. Microbiol. 41: 144-148 [Abstract] [Full Text]  
  • Richardson, D. C., Ciach, M., Zhong, K. J. Y., Crandall, I., Kain, K. C. (2002). Evaluation of the Makromed Dipstick Assay versus PCR for Diagnosis of Plasmodium falciparum Malaria in Returned Travelers. J. Clin. Microbiol. 40: 4528-4530 [Abstract] [Full Text]  
  • Zhong, K. J. Y., Salas, C. J., Shafer, R., Gubanov, A., Gasser, R. A. Jr., Magill, A. J., Forney, J. R., Kain, K. C. (2001). Comparison of IsoCode STIX and FTA Gene Guard Collection Matrices as Whole-Blood Storage and Processing Devices for Diagnosis of Malaria by PCR. J. Clin. Microbiol. 39: 1195-1196 [Abstract] [Full Text]  
  • Crandall, I., Charuk, J., Kain, K. C. (2000). Nonylphenolethoxylates as Malarial Chloroquine Resistance Reversal Agents. Antimicrob. Agents Chemother. 44: 2431-2434 [Abstract] [Full Text]  
  • Louie, M., Louie, L., Simor, A. E. (2000). The role of DNA amplification technology in the diagnosis of infectious diseases. CMAJ 163: 301-309 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»