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Journal of Clinical Microbiology, February 1999, p. 342-349, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Genotyping of Staphylococcus aureus Strains: Comparison of Repetitive Element Sequence-Based PCR with Various Typing Methods and Isolation of a Novel Epidemicity Marker

Anneke van der Zee,1,* Harold Verbakel,1 Johan-Carlo van Zon,1 Ine Frenay,2 Alex van Belkum,3 Marcel Peeters,1 Anton Buiting,1 and Anneke Bergmans1

Laboratory of Medical Microbiology, St. Elisabeth Hospital, 5000 AS Tilburg,1 Regional Laboratory of Medical Microbiology, 3300 AW Dordrecht,2 and Erasmus Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, 3015 GD Rotterdam,3 The Netherlands

Received 28 April 1998/Returned for modification 12 June 1998/Accepted 2 November 1998

Repetitive sequence-based (Rep)-PCR genotyping as described here is based on the presence of homologues of Mycoplasma pneumoniae repeat-like elements in Staphylococcus. In this study we comparatively evaluated the usefulness of rep-PCR typing with two sets of well-defined collections of Staphylococcus aureus strains. Rep-PCR analysis of the first collection of S. aureus strains (n = 59) and one Staphylococcus intermedius strain showed 14 different rep-PCR patterns, with each pattern harboring 6 to 15 DNA fragments. The discriminatory power of rep-PCR typing compared well to those of arbitrarily primed PCR (average of 20 types) and pulsed-field gel electrophoresis (11 types). S. aureus strain collection I comprised four outbreak-related groups of isolates. The isolates in only one group were found to have identical rep-PCR profiles. However, in an analysis of isolates from three additional independent local outbreaks (n for outbreaks 1 and 2 = 5, n for outbreak 3 = 12), identical rep-PCR types were found among strains isolated during each outbreak. Therefore, we conclude that rep-PCR genotyping may be an easy and fast method for monitoring of the epidemiology of nosocomial Staphylococcus infections. Rep-PCR analysis of strain collection II, which consisted of epidemic and nonepidemic methicillin-resistant S. aureus (MRSA) strains, revealed that a cluster of similar rep-PCR profiles was found among MRSA isolates which were more frequently isolated and which were most often associated with outbreaks.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands. Phone: 31 13 539 2676. Fax: 31 13 544 1264. E-mail: lab.med.microbiol{at}inter.NL.net.

Journal of Clinical Microbiology, February 1999, p. 342-349, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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