This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van der Zee, A.
Right arrow Articles by Bergmans, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Zee, A.
Right arrow Articles by Bergmans, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 342-349, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Genotyping of Staphylococcus aureus Strains: Comparison of Repetitive Element Sequence-Based PCR with Various Typing Methods and Isolation of a Novel Epidemicity Marker

Anneke van der Zee,1,* Harold Verbakel,1 Johan-Carlo van Zon,1 Ine Frenay,2 Alex van Belkum,3 Marcel Peeters,1 Anton Buiting,1 and Anneke Bergmans1

Laboratory of Medical Microbiology, St. Elisabeth Hospital, 5000 AS Tilburg,1 Regional Laboratory of Medical Microbiology, 3300 AW Dordrecht,2 and Erasmus Medical Center Rotterdam, Department of Medical Microbiology and Infectious Diseases, 3015 GD Rotterdam,3 The Netherlands

Received 28 April 1998/Returned for modification 12 June 1998/Accepted 2 November 1998

Repetitive sequence-based (Rep)-PCR genotyping as described here is based on the presence of homologues of Mycoplasma pneumoniae repeat-like elements in Staphylococcus. In this study we comparatively evaluated the usefulness of rep-PCR typing with two sets of well-defined collections of Staphylococcus aureus strains. Rep-PCR analysis of the first collection of S. aureus strains (n = 59) and one Staphylococcus intermedius strain showed 14 different rep-PCR patterns, with each pattern harboring 6 to 15 DNA fragments. The discriminatory power of rep-PCR typing compared well to those of arbitrarily primed PCR (average of 20 types) and pulsed-field gel electrophoresis (11 types). S. aureus strain collection I comprised four outbreak-related groups of isolates. The isolates in only one group were found to have identical rep-PCR profiles. However, in an analysis of isolates from three additional independent local outbreaks (n for outbreaks 1 and 2 = 5, n for outbreak 3 = 12), identical rep-PCR types were found among strains isolated during each outbreak. Therefore, we conclude that rep-PCR genotyping may be an easy and fast method for monitoring of the epidemiology of nosocomial Staphylococcus infections. Rep-PCR analysis of strain collection II, which consisted of epidemic and nonepidemic methicillin-resistant S. aureus (MRSA) strains, revealed that a cluster of similar rep-PCR profiles was found among MRSA isolates which were more frequently isolated and which were most often associated with outbreaks.

* Corresponding author. Mailing address: Laboratory of Medical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands. Phone: 31 13 539 2676. Fax: 31 13 544 1264. E-mail: lab.med.microbiol{at}inter.NL.net.

Journal of Clinical Microbiology, February 1999, p. 342-349, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pourcel, C., Hormigos, K., Onteniente, L., Sakwinska, O., Deurenberg, R. H., Vergnaud, G. (2009). Improved Multiple-Locus Variable-Number Tandem-Repeat Assay for Staphylococcus aureus Genotyping, Providing a Highly Informative Technique Together with Strong Phylogenetic Value. J. Clin. Microbiol. 47: 3121-3128 [Abstract] [Full Text]  
  • Sabat, A., Malachowa, N., Miedzobrodzki, J., Hryniewicz, W. (2006). Comparison of PCR-Based Methods for Typing Staphylococcus aureus Isolates.. J. Clin. Microbiol. 44: 3804-3807 [Abstract] [Full Text]  
  • Singh, A., Goering, R. V., Simjee, S., Foley, S. L., Zervos, M. J. (2006). Application of Molecular Techniques to the Study of Hospital Infection. Clin. Microbiol. Rev. 19: 512-530 [Abstract] [Full Text]  
  • van der Zee, A., Heck, M., Sterks, M., Harpal, A., Spalburg, E., Kazobagora, L., Wannet, W. (2005). Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR-Amplified Fragment Length Polymorphism Analysis of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 6042-6047 [Abstract] [Full Text]  
  • Pancholi, P., Healy, M., Bittner, T., Webb, R., Wu, F., Aiello, A., Larson, E., Latta, P. D. (2005). Molecular Characterization of Hand Flora and Environmental Isolates in a Community Setting. J. Clin. Microbiol. 43: 5202-5207 [Abstract] [Full Text]  
  • Francois, P., Huyghe, A., Charbonnier, Y., Bento, M., Herzig, S., Topolski, I., Fleury, B., Lew, D., Vaudaux, P., Harbarth, S., van Leeuwen, W., van Belkum, A., Blanc, D. S., Pittet, D., Schrenzel, J. (2005). Use of an Automated Multiple-Locus, Variable-Number Tandem Repeat-Based Method for Rapid and High-Throughput Genotyping of Staphylococcus aureus Isolates. J. Clin. Microbiol. 43: 3346-3355 [Abstract] [Full Text]  
  • Shutt, C. K., Pounder, J. I., Page, S. R., Schaecher, B. J., Woods, G. L. (2005). Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive-Sequence-Based PCR for Characterization of Staphylococcus aureus Strains. J. Clin. Microbiol. 43: 1187-1192 [Abstract] [Full Text]  
  • Wilck, M. B., Wu, Y., Howe, J. G., Crouch, J. Y., Edberg, S. C. (2001). Endocarditis Caused by Culture-Negative Organisms Visible by Brown and Brenn Staining: Utility of PCR and DNA Sequencing for Diagnosis. J. Clin. Microbiol. 39: 2025-2027 [Abstract] [Full Text]  
  • van Leeuwen, W., Libregts, C., Schalk, M., Veuskens, J., Verbrugh, H., van Belkum, A. (2001). Binary Typing of Staphylococcus aureus Strains through Reversed Hybridization Using Digoxigenin-Universal Linkage System-Labeled Bacterial Genomic DNA. J. Clin. Microbiol. 39: 328-331 [Abstract] [Full Text]  
  • Deplano, A., Schuermans, A., Van Eldere, J., Witte, W., Meugnier, H., Etienne, J., Grundmann, H., Jonas, D., Noordhoek, G. T., Dijkstra, J., van Belkum, A., van Leeuwen, W., Tassios, P. T., Legakis, N. J., van der Zee, A., Bergmans, A., Blanc, D. S., Tenover, F. C., Cookson, B. C., O'Neil, G., Struelens, M. J., The European Study Group on Epidemiological Marker, (2000). Multicenter Evaluation of Epidemiological Typing of Methicillin-Resistant Staphylococcus aureus Strains by Repetitive-Element PCR Analysis. J. Clin. Microbiol. 38: 3527-3533 [Abstract] [Full Text]  
  • Cramton, S. E., Schnell, N. F., Gotz, F., Bruckner, R. (2000). Identification of a New Repetitive Element in Staphylococcus aureus. Infect. Immun. 68: 2344-2348 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»