This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chatellier, S.
Right arrow Articles by Harel, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chatellier, S.
Right arrow Articles by Harel, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 362-366, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Relatedness of Streptococcus suis Serotype 2 Isolates from Different Geographic Origins as Evaluated by Molecular Fingerprinting and Phenotyping

Sonia Chatellier,1,dagger Marcelo Gottschalk,1 Robert Higgins,1 Roland Brousseau,2 and Josee Harel1,*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Québec, Canada J2S 7C6,1 and Biotechnology Research Institute, Montreal, Québec, Canada H4P 2R22

Received 7 August 1998/Returned for modification 2 October 1998/Accepted 16 November 1998

The genetic diversity of 88 Streptococcus suis serotype 2 isolates which were recovered from various countries was examined by randomly amplified polymorphic DNA (RAPD) analysis with three primers. This bacterial collection included 80 isolates of porcine origin and 8 of human origin. This investigation allowed the identification of 23 RAPD types containing 1 to 30 isolates originating from one to six countries. Common RAPD patterns were found between human and pig isolates. The isolates were also tested for the production of virulent factors such as hemolysin, muramidase-released protein (MRP), and extracellular factor (EF). All isolates exhibiting the virulent phenotype hemolysin+ MRP+ EF+ clearly clustered on the basis of fingerprinting by RAPD analysis. In a similar way, most of isolates with the hemolysin- MRP- EF- phenotype were assigned to one RAPD cluster. Therefore, RAPD clusters are more related to the phenotype defined with hemolysin, MRP, and EF than to the geographic origin of the isolates. These data indicate that RAPD analysis used in conjunction with phenotypic methods provides a reliable method for the assessment of the clonal relationship between S. suis isolates responsible for infections in pigs or humans, especially for those exhibiting the classic "virulent" phenotype hemolysin+ MRP+ EF+.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Québec, Canada J2S 7C6. Phone: (450) 773-8521, ext. 8233. Fax: (450) 778-8108. E-mail: harelj{at}ere.umontreal.ca.

dagger Present address: Research Service, Veterans Affairs Medical Center, Memphis, TN 38104.

Journal of Clinical Microbiology, February 1999, p. 362-366, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Baums, C. G., Verkuhlen, G. J., Rehm, T., Silva, L. M. G., Beyerbach, M., Pohlmeyer, K., Valentin-Weigand, P. (2007). Prevalence of Streptococcus suis Genotypes in Wild Boars of Northwestern Germany. Appl. Environ. Microbiol. 73: 711-717 [Abstract] [Full Text]  
  • Jobin, M.-C., Martinez, G., Motard, J., Gottschalk, M., Grenier, D. (2005). Cloning, Purification, and Enzymatic Properties of Dipeptidyl Peptidase IV from the Swine Pathogen Streptococcus suis. J. Bacteriol. 187: 795-799 [Abstract] [Full Text]  
  • Vanier, G., Segura, M., Friedl, P., Lacouture, S., Gottschalk, M. (2004). Invasion of Porcine Brain Microvascular Endothelial Cells by Streptococcus suis Serotype 2. Infect. Immun. 72: 1441-1449 [Abstract] [Full Text]  
  • Harel, J., Martinez, G., Nassar, A., Dezfulian, H., Labrie, S. J., Brousseau, R., Moineau, S., Gottschalk, M. (2003). Identification of an Inducible Bacteriophage in a Virulent Strain of Streptococcus suis Serotype 2. Infect. Immun. 71: 6104-6108 [Abstract] [Full Text]  
  • Vela, A. I., Goyache, J., Tarradas, C., Luque, I., Mateos, A., Moreno, M. A., Borge, C., Perea, J. A., Dominguez, L., Fernandez-Garayzabal, J. F. (2003). Analysis of Genetic Diversity of Streptococcus suis Clinical Isolates from Pigs in Spain by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 41: 2498-2502 [Abstract] [Full Text]  
  • Facklam, R. (2002). What Happened to the Streptococci: Overview of Taxonomic and Nomenclature Changes. Clin. Microbiol. Rev. 15: 613-630 [Abstract] [Full Text]  
  • King, S. J., Leigh, J. A., Heath, P. J., Luque, I., Tarradas, C., Dowson, C. G., Whatmore, A. M. (2002). Development of a Multilocus Sequence Typing Scheme for the Pig Pathogen Streptococcus suis: Identification of Virulent Clones and Potential Capsular Serotype Exchange. J. Clin. Microbiol. 40: 3671-3680 [Abstract] [Full Text]  
  • de Greeff, A., Buys, H., Verhaar, R., van Alphen, L., Smith, H. E. (2002). Distribution of Environmentally Regulated Genes of Streptococcus suis Serotype 2 among S. suis Serotypes and Other Organisms. J. Clin. Microbiol. 40: 3261-3268 [Abstract] [Full Text]  
  • Segura, M., Gottschalk, M. (2002). Streptococcus suis Interactions with the Murine Macrophage Cell Line J774: Adhesion and Cytotoxicity. Infect. Immun. 70: 4312-4322 [Abstract] [Full Text]  
  • Takamatsu, D., Osaki, M., Sekizaki, T. (2002). Evidence for Lateral Transfer of the Suilysin Gene Region of Streptococcus suis. J. Bacteriol. 184: 2050-2057 [Abstract] [Full Text]  
  • Berthelot-Herault, F., Marois, C., Gottschalk, M., Kobisch, M. (2002). Genetic Diversity of Streptococcus suis Strains Isolated from Pigs and Humans as Revealed by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 615-619 [Abstract] [Full Text]  
  • Sekizaki, T., Osaki, M., Takamatsu, D., Shimoji, Y. (2001). Distribution of the SsuDAT1I Restriction-Modification System among Different Serotypes of Streptococcus suis. J. Bacteriol. 183: 5436-5440 [Abstract] [Full Text]  
  • Smith, H. E., Buijs, H., Wisselink, H. J., Stockhofe-Zurwieden, N., Smits, M. A. (2001). Selection of Virulence-Associated Determinants of Streptococcus suis Serotype 2 by In Vivo Complementation. Infect. Immun. 69: 1961-1966 [Abstract] [Full Text]  
  • Allgaier, A., Goethe, R., Wisselink, H. J., Smith, H. E., Valentin-Weigand, P. (2001). Relatedness of Streptococcus suis Isolates of Various Serotypes and Clinical Backgrounds as Evaluated by Macrorestriction Analysis and Expression of Potential Virulence Traits. J. Clin. Microbiol. 39: 445-453 [Abstract] [Full Text]  
  • Lalonde, M., Segura, M., Lacouture, S., Gottschalk, M. (2000). Interactions between Streptococcus suis serotype 2 and different epithelial cell lines. Microbiology 146: 1913-1921 [Abstract] [Full Text]  
  • Charland, N., Nizet, V., Rubens, C. E., Kim, K. S., Lacouture, S., Gottschalk, M. (2000). Streptococcus suis Serotype 2 Interactions with Human Brain Microvascular Endothelial Cells. Infect. Immun. 68: 637-643 [Abstract] [Full Text]  
  • Gottschalk, M., Higgins, R., Quessy, S. (1999). Dilemma of the Virulence of Streptococcus suis Strains. J. Clin. Microbiol. 37: 4202-4203 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»