This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmid, D. S.
Right arrow Articles by Reeves, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmid, D. S.
Right arrow Articles by Reeves, W. C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 376-379, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

D. Scott Schmid,1,* Denise R. Brown,1 Rosane Nisenbaum,2 Rae Lyn Burke,3 D'Anna Alexander,3 Rhoda Ashley,4 Philip E. Pellett,1 and William C. Reeves1

Division of Viral and Rickettsial Diseases, Viral Exanthems and Herpesviruses Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331; Klemm Analysis Group, Atlanta, Georgia 303452; Chiron Vaccines, Chiron Corporation, Emeryville, California 946083; and Children's Hospital, University of Washington, Seattle, Washington 981054

Received 20 August 1998/Returned for modification 7 October 1998/Accepted 18 November 1998

Type-specific serologic assays for herpes simplex virus (HSV) types 1 and 2 based on glycoprotein G-1 (gG-1) (HSV-1) and gG-2 (HSV-2) discriminate between antibodies against HSV-1 and HSV-2. We previously developed a Western blot assay using gG-1 and gG-2 expressed in baculovirus, performed extensive validation studies, and determined that it was both sensitive and specific for type-specific detection of HSV antibody. Here we report that, among a cohort of Thai military recruits, the serostatus of some individuals changed from positive to negative over time (6.6% among those ever positive for HSV-1, and 14.9% among those ever positive for HSV-2). We tested a subset of these specimens in three other gG-based assays: an enzyme-linked immunosorbent assay, an immunoblot strip assay, and a Western blot assay. Positive-to-negative shifts occurred in every assay; the frequency of the shifts ranged from 6.1% to 21.2% of the specimen sets tested. There was only limited agreement among the assays concerning which individuals lost reactivity. This inaccuracy, exhibited by all of the assay protocols, was not predicted by validation studies employing specimens from cross-sectional studies and was most pronounced in HSV-2 testing. This argues for the inclusion of serial blood specimens in serologic assay validation procedures.


* Corresponding author. Mailing address: CDC, NCID, Division of Viral and Rickettial Diseases, Viral Exanthems and Herpesvirus Branch, 1600 Clifton Rd., MSD-10, Atlanta GA 30333. Phone: (404) 639-0066. Fax: (404) 639-4056. E-mail: dss1{at}cdc.gov.


Journal of Clinical Microbiology, February 1999, p. 376-379, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Chentoufi, A. A., Binder, N. R., Berka, N., Durand, G., Nguyen, A., Bettahi, I., Maillere, B., BenMohamed, L. (2008). Asymptomatic Human CD4+ Cytotoxic T-Cell Epitopes Identified from Herpes Simplex Virus Glycoprotein B. J. Virol. 82: 11792-11802 [Abstract] [Full Text]  
  • Zhang, X., Castelli, F. A., Zhu, X., Wu, M., Maillere, B., BenMohamed, L. (2008). Gender-Dependent HLA-DR-Restricted Epitopes Identified from Herpes Simplex Virus Type 1 Glycoprotein D. CVI 15: 1436-1449 [Abstract] [Full Text]  
  • Chentoufi, A. A., Zhang, X., Lamberth, K., Dasgupta, G., Bettahi, I., Nguyen, A., Wu, M., Zhu, X., Mohebbi, A., Buus, S., Wechsler, S. L., Nesburn, A. B., BenMohamed, L. (2008). HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D. J. Immunol. 180: 426-437 [Abstract] [Full Text]  
  • Pebody, R G, Andrews, N, Brown, D, Gopal, R, de Melker, H, Francois, G, Gatcheva, N, Hellenbrand, W, Jokinen, S, Klavs, I, Kojouharova, M, Kortbeek, T, Kriz, B, Prosenc, K, Roubalova, K, Teocharov, P, Thierfelder, W, Valle, M, Van Damme, P, Vranckx, R (2004). The seroepidemiology of herpes simplex virus type 1 and 2 in Europe. Sex. Transm. Infect. 80: 185-191 [Abstract] [Full Text]  
  • Wales, S. Q., Smith, C. C., Wachsman, M., Calton, G., Aurelian, L. (2004). Performance and Use of a Ribonucleotide Reductase Herpes Simplex Virus Type-Specific Serological Assay. CVI 11: 42-49 [Abstract] [Full Text]  
  • Gorander, S., Svennerholm, B., Liljeqvist, J.-A. (2003). Secreted Portion of Glycoprotein G of Herpes Simplex Virus Type 2 Is a Novel Antigen for Type-Discriminating Serology. J. Clin. Microbiol. 41: 3681-3686 [Abstract] [Full Text]  
  • Lai, W, Chen, C Y, Morse, S A, Htun, Y., Fehler, H G, Liu, H, Ballard, R C (2003). Increasing relative prevalence of HSV-2 infection among men with genital ulcers from a mining community in South Africa. Sex. Transm. Infect. 79: 202-207 [Abstract] [Full Text]  
  • Cherpes, T. L., Ashley, R. L., Meyn, L. A, Hillier, S. L. (2003). Longitudinal Reliability of Focus Glycoprotein G-Based Type-Specific Enzyme Immunoassays for Detection of Herpes Simplex Virus Types 1 and 2 in Women. J. Clin. Microbiol. 41: 671-674 [Abstract] [Full Text]  
  • Fisman, D N, Hook, E W III, Goldie, S J (2003). Estimating the costs and benefits of screening monogamous, heterosexual couples for unrecognised infection with herpes simplex virus type 2. Sex. Transm. Infect. 79: 45-52 [Abstract] [Full Text]  
  • Ikoma, M., Liljeqvist, J.-A., Groen, J., Glazenburg, K. L., The, T. H., Welling-Wester, S. (2002). Use of a Fragment of Glycoprotein G-2 Produced in the Baculovirus Expression System for Detecting Herpes Simplex Virus Type 2-Specific Antibodies. J. Clin. Microbiol. 40: 2526-2532 [Abstract] [Full Text]  
  • Eing, B. R., Lippelt, L., Lorentzen, E. U., Hafezi, W., Schlumberger, W., Steinhagen, K., Kuhn, J. E. (2002). Evaluation of Confirmatory Strategies for Detection of Type-Specific Antibodies against Herpes Simplex Virus Type 2. J. Clin. Microbiol. 40: 407-413 [Abstract] [Full Text]  
  • Liljeqvist, J.-A., Trybala, E., Hoebeke, J., Svennerholm, B., Bergstrom, T. (2002). Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants. J. Gen. Virol. 83: 157-165 [Abstract] [Full Text]  
  • Ashley, R. L (2001). Sorting out the new HSV type specific antibody tests. Sex. Transm. Infect. 77: 232-237 [Abstract] [Full Text]  
  • Armstrong, G. L., Schillinger, J., Markowitz, L., Nahmias, A. J., Johnson, R. E., McQuillan, G. M., St. Louis, M. E. (2001). Incidence of Herpes Simplex Virus Type 2 Infection in the United States. Am J Epidemiol 153: 912-920 [Abstract] [Full Text]  
  • Van Doornum, G. J. J., Slomka, M. J., Buimer, M., Groen, J., Van den Hoek, J. A. R., Cairo, I., Vyse, A., Brown, D. W. G. (2000). Comparison of a Monoclonal Antibody-Blocking Enzyme-Linked Immunoassay and a Strip Immunoblot Assay for Identifying Type-Specific Herpes Simplex Virus Type 2 Serological Responses. CVI 7: 641-644 [Abstract] [Full Text]  
  • Rekabdar, E., Tunback, P., Liljeqvist, J.-A., Bergstrom, T. (1999). Variability of the Glycoprotein G Gene in Clinical Isolates of Herpes Simplex Virus Type 1. CVI 6: 826-831 [Abstract] [Full Text]