Journal of Clinical Microbiology, February 1999, p. 391-395, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Enteric and Respiratory Virus Laboratory,
Received 25 June 1998/Returned for modification 2 September
1998/Accepted 9 October 1998
An immunoglobulin G (IgG)-capture enzyme-linked immunosorbent
assay (ELISA) for rubella virus is described. The assay uses a
fluorescein isothiocyanate (FITC)-anti-FITC amplification system. The
detection limit of the ELISA was approximately 7 IU of rubella virus-specific IgG per ml of serum sample. For saliva samples the
performances of the capture ELISA and previously described radioimmunoassay were assessed, and the results of those two assays were compared to the rubella virus-specific IgG result obtained by a
commercial ELISA (Behring Enzygnost) with a panel of paired serum and
saliva samples. This comparison showed that the capture ELISA with
saliva was more sensitive than the radioimmunoassay and that the
results correlated better with the serum IgG result than the results of
the radioimmunoassay did, with an overall sensitivity of 82% and a
rank correlation of 0.68, whereas the sensitivity and rank correlation
for the radioimmunoassay were 74% and 0.45, respectively. For subjects
of 10 years of age or younger, the ELISA with saliva had a sensitivity
of 94% and a specificity of 100% compared to the results of the ELISA
(Behring Enzygnost) for rubella virus-specific IgG with corresponding
serum samples. The sensitivity was much lower for subjects ages 17 years or older. The assay may have wider epidemiological use with
saliva specimens, particularly those from children.
*
Corresponding author. Mailing address: Enteric and
Respiratory Virus Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 181 2004400. Fax: 44 181 2001569. E-mail: avyse{at}phls.co.uk.
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