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Journal of Clinical Microbiology, February 1999, p. 427-429, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Test Characteristics of Acridine Orange, Gram, and May-Grünwald-Giemsa Stains for Enumeration of Intracellular Organisms in Bronchoalveolar Lavage Fluid

Els De Brauwer,1 Jan Jacobs,1,* Fred Nieman,2 Cathrien Bruggeman,1 and Marjolein Drent3

Departments of Medical Microbiology,1 Patient Research,2 and Pulmonology,3 University Hospital Maastricht, The Netherlands

Received 16 June 1998/Returned for modification 26 August 1998/Accepted 28 October 1998

For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grünwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Hospital Maastricht, P.O. Box 5800, Maastricht 6202 AZ, The Netherlands. Phone: 31 43 3876644. Fax: 31 43 3876643. E-mail: JJA{at}lmib.azm.nl.

Journal of Clinical Microbiology, February 1999, p. 427-429, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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