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Journal of Clinical Microbiology, February 1999, p. 438-441, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Baculovirus Expression of Chimeric Hepatitis B Virus Core Particles with Hepatitis E Virus Epitopes and Their Use in a Hepatitis E Immunoassay

Antoine Touze,1 Nathalie Enogat,1 Yves Buisson,2 and Pierre Coursaget1,*

Institut de Virologie de Tours and Laboratoire des Processus Infectieux et Tumoraux, Faculté des Sciences Pharmaceutiques "Philippe Maupas," 37200 Tours,1 and Laboratoire de Biologie Clinique, Hôpital d'Instruction des Armées du Val de Grâce, Paris,2 France

Received 26 May 1998/Returned for modification 9 October 1998/Accepted 18 November 1998

Two hepatitis B core proteins bearing the immunodominant region of the hepatitis E virus (HEV) capsid protein, one at the C terminus of hepatitis B virus core antigen (HBcAg) and the other within the HBcAg immunodominant loop, were constructed. Both chimeric proteins exhibited HEV reactivity, but only the first construct retained HBcAg reactivity. The second construct was used to develop an anti-HEV test which is equivalent to a commercial test for the detection of anti-HEV immunoglobulin G (IgG) but is more sensitive for the detection of anti-HEV IgM.

* Corresponding author. Mailing address: Institut de Virologie de Tours and Laboratoire des Processus Infectieux et Tumoraux, Faculté des Sciences Pharmaceutiques "Philippe Maupas," 31 avenue Monge, 37200 Tours, France. Phone: 33 2 47 36 72 56. Fax: 33 2 47 36 71 88. E-mail: coursaget{at}univ-tours.fr.

Journal of Clinical Microbiology, February 1999, p. 438-441, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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