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Journal of Clinical Microbiology, February 1999, p. 442-444, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Stability of Insertion Sequence IS1245, a Marker for Differentiation of Mycobacterium avium Strains

Jeanett Bauer* and Åse Bengård Andersen

Department of Mycobacteriology, Statens Serum Institut, 2300 Copenhagen S, Denmark

Received 29 July 1998/Returned for modification 22 September 1998/Accepted 23 October 1998

Recently a novel insertion element, IS1245, has been described and suggested for use as a probe in restriction fragment length polymorphism studies of Mycobacterium avium strains. An important issue in this context is the stability of the insertion element. We analyzed single colonies of M. avium cultures and found frequent small one- to two-band changes. However, following repeated in vitro passages over 1 year, similar one- to two-band changes were observed in the IS1245 patterns of only six M. avium strains investigated.

* Corresponding author. Mailing address: Department of Mycobacteriology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Phone: 45 3268 3705. Fax: 45 3268 3871. E-mail: jba{at}ssi.dk.

Journal of Clinical Microbiology, February 1999, p. 442-444, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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