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Journal of Clinical Microbiology, February 1999, p. 447-449, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Comparison of NucliSens and Roche Monitor Assays
for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma
John R.
Dyer,1,
Christopher D.
Pilcher,1
Robin
Shepard,2
Jody
Schock,2
Joseph J.
Eron,1 and
Susan A.
Fiscus2,*
Departments of
Medicine1 and
Microbiology and
Immunology,2 The University of North
Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Received 22 July 1998/Returned for modification 9 September
1998/Accepted 1 October 1998
We compared the performance of Organon Teknika's NucliSens and
Roche Diagnostic Systems' Monitor quantitative human immunodeficiency type 1 RNA assays. Both had similar linearity and sensitivity over most
of the dynamic range of the assays, although the Monitor assay was
superior at the low range of RNA values while the NucliSens assay was
more consistent at higher RNA values. NucliSens generally showed less
interassay variability.
*
Corresponding author. Mailing address: University of
North Carolina at Chapel Hill, CB# 7140, Chapel Hill, NC 27599-7140. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail:
sfiscus{at}emailunc.edu.

Present address: Ipswich General Hospital, Ipswich 4305, Queensland,
Australia.
Journal of Clinical Microbiology, February 1999, p. 447-449, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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