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Journal of Clinical Microbiology, February 1999, p. 447-449, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of NucliSens and Roche Monitor Assays for Quantitation of Levels of Human Immunodeficiency Virus Type 1 RNA in Plasma

John R. Dyer,1,dagger Christopher D. Pilcher,1 Robin Shepard,2 Jody Schock,2 Joseph J. Eron,1 and Susan A. Fiscus2,*

Departments of Medicine1 and Microbiology and Immunology,2 The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599

Received 22 July 1998/Returned for modification 9 September 1998/Accepted 1 October 1998

We compared the performance of Organon Teknika's NucliSens and Roche Diagnostic Systems' Monitor quantitative human immunodeficiency type 1 RNA assays. Both had similar linearity and sensitivity over most of the dynamic range of the assays, although the Monitor assay was superior at the low range of RNA values while the NucliSens assay was more consistent at higher RNA values. NucliSens generally showed less interassay variability.


* Corresponding author. Mailing address: University of North Carolina at Chapel Hill, CB# 7140, Chapel Hill, NC 27599-7140. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: sfiscus{at}emailunc.edu.

dagger Present address: Ipswich General Hospital, Ipswich 4305, Queensland, Australia.


Journal of Clinical Microbiology, February 1999, p. 447-449, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1999 by the American Society for Microbiology. All rights reserved.