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Journal of Clinical Microbiology, February 1999, p. 450-452, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Randomly Amplified Polymorphic DNA Analysis and Pulsed-Field Gel Electrophoresis for Typing of Moraxella catarrhalis Strains

Hoang Vu-Thien,1,* Carole Dulot,1 Didier Moissenet,1 Brigitte Fauroux,2 and Antoine Garbarg-Chenon1

Service de Microbiologie1 and Service de Pédiatrie et Pneumologie,2 Hôpital d'Enfants Armand-Trousseau, 75571 Paris Cedex 12, France

Received 11 May 1998/Returned for modification 28 September 1998/Accepted 2 November 1998

Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing of M. catarrhalis.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, 75571 Paris Cedex 12, France. Phone: (33) 144 73 61 43. Fax: (33) 144 73 62 88. E-mail: hoang.vuthien{at}trs.ap-hop-paris.fr.

Journal of Clinical Microbiology, February 1999, p. 450-452, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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