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Journal of Clinical Microbiology, February 1999, p. 453-456, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Quantitation of Human Immunodeficiency Virus Type 2 DNA in Peripheral Blood Mononuclear Cells by Using a Quantitative-Competitive PCR Assay

Perpétua Gomes,1,2 Nuno Costa Taveira,1,2 José Moniz Pereira,1 Francisco Antunes,3 M. O. Santos Ferreira,1 and Maria Helena Lourenço1,*

Departamento de Microbiologia, Faculdade de Farmácia, Universidade de Lisboa,1 and Serviço de Doenças Infecto-Contagiosas, Hospital de Santa Maria,3 Lisbon, and Instituto Superior de Ciências da Saúde, Travessa da Granja, Monte da Caparica,2 Portugal

Received 10 June 1998/Returned for modification 6 October 1998/Accepted 18 November 1998

A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the proviral load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and proviral loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.

* Corresponding author. Mailing address: Faculdade de Farmácia da Universidade de Lisboa, Departamento de Microbiologia, Avenida das Forças Armadas, 1600 Lisbon, Portugal. Phone: 351.1.7934212. Fax: 351.1.7934212. E-mail: mhlourenco{at}ff.ul.pt.

Journal of Clinical Microbiology, February 1999, p. 453-456, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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