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Journal of Clinical Microbiology, February 1999, p. 457-460, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Cryptosporidium parvum in Horses: Thresholds of Acid-Fast Stain, Immunofluorescence Assay, and Flow Cytometry

D. J. Cole,1,* K. Snowden,2 N. D. Cohen,1 and R. Smith2

Department of Large Animal Medicine and Surgery1 and Department of Veterinary Pathobiology,2 College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4475

Received 9 April 1998/Returned for modification 1 May 1998/Accepted 30 October 1998

Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (101 to 106/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 × 105 oocysts/g of feces for the IFA and AF techniques and 5 × 104 oocysts/g for flow cytometry.

* Corresponding author. Present address: Department of Epidemiology, School of Public Health, University of North Carolina, CB #7400 McGavran-Greenberg Hall, Chapel Hill, NC 27599-7400. Phone: (919) 969-6747. Fax: (919) 966-2089. E-mail: dcole{at}sph.unc.edu.

Journal of Clinical Microbiology, February 1999, p. 457-460, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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