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Journal of Clinical Microbiology, February 1999, p. 464-466, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Gram Type-Specific Broad-Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria

Alfred Klausegger,1 Markus Hell,2 Alexandra Berger,1 Kerstin Zinober,1 Sabine Baier,1 Neil Jones,1 Wolfgang Sperl,1 and Barbara Kofler1,*

Children's Hospital1 and Institute of Bacteriology,2 General Hospital Salzburg, Salzburg, Austria

Received 11 June 1998/Returned for modification 22 July 1998/Accepted 2 November 1998

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

* Corresponding author. Mailing address: Children's Hospital, General Hospital Salzburg, Müllner Hauptstr. 48, A-5020 Salzburg, Austria. Phone: 43 662 4482 2650. Fax: 43 662 4482 2604. E-mail: b.kofler{at}lkasbg.gv.at.

Journal of Clinical Microbiology, February 1999, p. 464-466, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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