JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klausegger, A.
Right arrow Articles by Kofler, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klausegger, A.
Right arrow Articles by Kofler, B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1999, p. 464-466, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Gram Type-Specific Broad-Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria

Alfred Klausegger,1 Markus Hell,2 Alexandra Berger,1 Kerstin Zinober,1 Sabine Baier,1 Neil Jones,1 Wolfgang Sperl,1 and Barbara Kofler1,*

Children's Hospital1 and Institute of Bacteriology,2 General Hospital Salzburg, Salzburg, Austria

Received 11 June 1998/Returned for modification 22 July 1998/Accepted 2 November 1998

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

* Corresponding author. Mailing address: Children's Hospital, General Hospital Salzburg, Müllner Hauptstr. 48, A-5020 Salzburg, Austria. Phone: 43 662 4482 2650. Fax: 43 662 4482 2604. E-mail: b.kofler{at}lkasbg.gv.at.

Journal of Clinical Microbiology, February 1999, p. 464-466, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1999 by the American Society for Microbiology. All rights reserved.