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Journal of Clinical Microbiology, February 1999, p. 471-474, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Retrospective Analysis of the Beijing Family of Mycobacterium tuberculosis in Preserved Lung Tissues

Lishi Qian,1 Jan D. A. Van Embden,2 Adri G. M. Van Der Zanden,3 Evert F. Weltevreden,4 Hongjin Duanmu,5 and James T. Douglas1,*

Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 968221; Laboratory of Infectious Disease, National Institute of Public Health and Environmental Protection, 3720 BA, Bilthoven,2 and Department of Microbiology and Infectious Diseases, Medical Laboratories,3 and Laboratory of Clinical Pathology,4 Hospital Center Apeldoorn, 7300 DS Apeldoorn, The Netherlands; and National Tuberculosis Control Center, Beijing 101149, People's Republic of China5

Received 16 July 1998/Returned for modification 23 July 1998/Accepted 9 November 1998

Direct repeat spoligotyping of 85 paraffin-embedded lung biopsies was used to investigated the occurrence around Beijing of the Beijing family of Mycobacterium tuberculosis. Samples ranged in time from 1956 to 1990. Hybridization patterns were found with 49 (58%) samples, and 45 (92%) produced typical Beijing family patterns extending over the 34-year period.

* Corresponding author. Mailing address: University of Hawaii at Manoa, Department of Microbiology, 2538 The Mall (Snyder Hall 101), Honolulu, HI 96822. Phone: (808) 956-4639. Fax: (808) 956-5339. E-mail: jdouglas{at}hawaii.edu.

Journal of Clinical Microbiology, February 1999, p. 471-474, Vol. 37, No. 2
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.