This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beltrán, P.
Right arrow Articles by Cravioto, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beltrán, P.
Right arrow Articles by Cravioto, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 581-590, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Diversity and Population Structure of Vibrio cholerae

Pilar Beltrán,1 Gabriela Delgado,1 Armando Navarro,1 Francisca Trujillo,1 Robert K. Selander,2,* and Alejandro Cravioto1

Departamento de Salud Pública de la Facultad de Medicina, Universidad Nacional Autónoma de México, México, D.F., México,1 and Institute of Molecular Evolutionary Genetics, Pennsylvania State University, University Park, Pennsylvania 168022

Received 9 September 1998/Returned for modification 17 November 1998/Accepted 8 December 1998

Multilocus enzyme electrophoresis (MLEE) of 397 Vibrio cholerae isolates, including 143 serogroup reference strains and 244 strains from Mexico and Guatemala, identified 279 electrophoretic types (ETs) distributed in two major divisions (I and II). Linkage disequilibrium was demonstrated in both divisions and in subdivision Ic of division I but not in subdivision Ia, which includes 76% of the ETs. Despite this evidence of relatively frequent recombination, clonal lineages may persist for periods of time measured in at least decades. In addition to the pandemic clones of serogroups O1 and O139, which form a tight cluster of four ETs in subdivision Ia, MLEE analysis identified numerous apparent clonal lineages of non-O1 strains with intercontinental distributions. A clone of serogroup O37 that demonstrated epidemic potential in the 1960s is closely related to the pandemic O1/O139 clones, but the nontoxigenic O1 Inaba El Tor reference strain is not. A strain of serogroup O22, which has been identified as the most likely donor of exogenous rfb region DNA to the O1 progenitor of the O139 clone, is distantly related to the O1/O139 clones. The close evolutionary relationships of the O1, O139, and O37 epidemic clones indicates that new cholera clones are likely to arise by the modification of a lineage that is already epidemic or is closely related to such a clone.

* Corresponding author. Mailing address: Institute of Molecular Evolutionary Genetics, Mueller Laboratory 516, Pennsylvania State University, University Park, PA 16802. Phone: (814) 234-8997. Fax: (814) 863-4706. E-mail: rks3{at}psu.edu.

Journal of Clinical Microbiology, March 1999, p. 581-590, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Talyzina, N. M., Ingvarsson, P. K., Zhu, J., Wai, S. N., Andersson, A. (2009). Molecular Diversification in the Quorum-Sensing System of Vibrio cholerae: Role of Natural Selection in the Emergence of Pandemic Strains. Appl. Environ. Microbiol. 75: 3808-3812 [Abstract] [Full Text]  
  • Lizarraga-Partida, M. L., Quilici, M.-L. (2009). Molecular Analyses of Vibrio cholerae O1 Clinical Strains, Including New Nontoxigenic Variants Isolated in Mexico during the Cholera Epidemic Years between 1991 and 2000. J. Clin. Microbiol. 47: 1364-1371 [Abstract] [Full Text]  
  • Zo, Y.-G., Chokesajjawatee, N., Grim, C., Arakawa, E., Watanabe, H., Colwell, R. R. (2009). Diversity and Seasonality of Bioluminescent Vibrio cholerae Populations in Chesapeake Bay. Appl. Environ. Microbiol. 75: 135-146 [Abstract] [Full Text]  
  • Chokesajjawatee, N., Zo, Y.-G., Colwell, R. R. (2008). Determination of Clonality and Relatedness of Vibrio cholerae Isolates by Genomic Fingerprinting, Using Long-Range Repetitive Element Sequence-Based PCR. Appl. Environ. Microbiol. 74: 5392-5401 [Abstract] [Full Text]  
  • Udden, S. M. N., Zahid, M. S. H., Biswas, K., Ahmad, Q. S., Cravioto, A., Nair, G. B., Mekalanos, J. J., Faruque, S. M. (2008). Acquisition of classical CTX prophage from Vibrio cholerae O141 by El Tor strains aided by lytic phages and chitin-induced competence. Proc. Natl. Acad. Sci. USA 105: 11951-11956 [Abstract] [Full Text]  
  • Pang, B., Yan, M., Cui, Z., Ye, X., Diao, B., Ren, Y., Gao, S., Zhang, L., Kan, B. (2007). Genetic Diversity of Toxigenic and Nontoxigenic Vibrio cholerae Serogroups O1 and O139 Revealed by Array-Based Comparative Genomic Hybridization. J. Bacteriol. 189: 4837-4849 [Abstract] [Full Text]  
  • Miller, M. C., Keymer, D. P., Avelar, A., Boehm, A. B., Schoolnik, G. K. (2007). Detection and Transformation of Genome Segments That Differ within a Coastal Population of Vibrio cholerae Strains. Appl. Environ. Microbiol. 73: 3695-3704 [Abstract] [Full Text]  
  • Danin-Poleg, Y., Cohen, L. A., Gancz, H., Broza, Y. Y., Goldshmidt, H., Malul, E., Valinsky, L., Lerner, L., Broza, M., Kashi, Y. (2007). Vibrio cholerae Strain Typing and Phylogeny Study Based on Simple Sequence Repeats. J. Clin. Microbiol. 45: 736-746 [Abstract] [Full Text]  
  • Schuenzel, E. L., Scally, M., Stouthamer, R., Nunney, L. (2005). A Multigene Phylogenetic Study of Clonal Diversity and Divergence in North American Strains of the Plant Pathogen Xylella fastidiosa. Appl. Environ. Microbiol. 71: 3832-3839 [Abstract] [Full Text]  
  • O'Shea, Y. A., Reen, F. J., Quirke, A. M., Boyd, E. F. (2004). Evolutionary Genetic Analysis of the Emergence of Epidemic Vibrio cholerae Isolates on the Basis of Comparative Nucleotide Sequence Analysis and Multilocus Virulence Gene Profiles. J. Clin. Microbiol. 42: 4657-4671 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Gutacker, M., Conza, N., Benagli, C., Pedroli, A., Bernasconi, M. V., Permin, L., Aznar, R., Piffaretti, J.-C. (2003). Population Genetics of Vibrio vulnificus: Identification of Two Divisions and a Distinct Eel-Pathogenic Clone. Appl. Environ. Microbiol. 69: 3203-3212 [Abstract] [Full Text]  
  • Kotetishvili, M., Stine, O. C., Chen, Y., Kreger, A., Sulakvelidze, A., Sozhamannan, S., Morris, J. G. Jr. (2003). Multilocus Sequence Typing Has Better Discriminatory Ability for Typing Vibrio cholerae than Does Pulsed-Field Gel Electrophoresis and Provides a Measure of Phylogenetic Relatedness. J. Clin. Microbiol. 41: 2191-2196 [Abstract] [Full Text]  
  • Faruque, S. M., Sack, D. A., Sack, R. B., Colwell, R. R., Takeda, Y., Nair, G. B. (2003). Inaugural Article: Emergence and evolution of Vibrio cholerae O139. Proc. Natl. Acad. Sci. USA 100: 1304-1309 [Abstract] [Full Text]  
  • Lipp, E. K., Huq, A., Colwell, R. R. (2002). Effects of Global Climate on Infectious Disease: the Cholera Model. Clin. Microbiol. Rev. 15: 757-770 [Abstract] [Full Text]  
  • Zo, Y.-G., Rivera, I. N. G., Russek-Cohen, E., Islam, M. S., Siddique, A. K., Yunus, M., Sack, R. B., Huq, A., Colwell, R. R. (2002). Genomic profiles of clinical and environmental isolates of Vibrio cholerae O1 in cholera-endemic areas of Bangladesh. Proc. Natl. Acad. Sci. USA 99: 12409-12414 [Abstract] [Full Text]  
  • Nair, G. B., Faruque, S. M., Bhuiyan, N. A., Kamruzzaman, M., Siddique, A. K., Sack, D. A. (2002). New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh. J. Clin. Microbiol. 40: 3296-3299 [Abstract] [Full Text]  
  • Boyd, E. F., Waldor, M. K. (2002). Evolutionary and functional analyses of variants of the toxin-coregulated pilus protein TcpA from toxigenic Vibrio cholerae non-O1/non-O139 serogroup isolates. Microbiology 148: 1655-1666 [Abstract] [Full Text]  
  • Farfan, M., Minana-Galbis, D., Fuste, M. C., Loren, J. G. (2002). Allelic Diversity and Population Structure in Vibrio cholerae O139 Bengal Based on Nucleotide Sequence Analysis. J. Bacteriol. 184: 1304-1313 [Abstract] [Full Text]  
  • Mukhopadhyay, A. K., Chakraborty, S., Takeda, Y., Nair, G. B., Berg, D. E. (2001). Characterization of VPI Pathogenicity Island and CTX{phi} Prophage in Environmental Strains of Vibrio cholerae. J. Bacteriol. 183: 4737-4746 [Abstract] [Full Text]  
  • Vieira, V. V., Teixeira, L. F. M., Vicente, A. C. P., Momen, H., Salles, C. A. (2001). Differentiation of Environmental and Clinical Isolates of Vibrio mimicus from Vibrio cholerae by Multilocus Enzyme Electrophoresis. Appl. Environ. Microbiol. 67: 2360-2364 [Abstract] [Full Text]  
  • Gándara, B., Merino, A. L., Rogel, M. A., Martínez-Romero, E. (2001). Limited Genetic Diversity of Brucella spp.. J. Clin. Microbiol. 39: 235-240 [Abstract] [Full Text]  
  • Stine, O. C., Sozhamannan, S., Gou, Q., Zheng, S., Morris, J. G. Jr., Johnson, J. A. (2000). Phylogeny of Vibrio cholerae Based on recA Sequence. Infect. Immun. 68: 7180-7185 [Abstract] [Full Text]  
  • Boyd, E. F., Heilpern, A. J., Waldor, M. K. (2000). Molecular Analyses of a Putative CTXphi Precursor and Evidence for Independent Acquisition of Distinct CTXphi s by Toxigenic Vibrio cholerae. J. Bacteriol. 182: 5530-5538 [Abstract] [Full Text]  
  • Farfán, M., Miñana, D., Fusté, M. C., Lorén, J. G. (2000). Genetic relationships between clinical and environmental Vibrio cholerae isolates based on multilocus enzyme electrophoresis. Microbiology 146: 2613-2626 [Abstract] [Full Text]  
  • Matsumoto, C., Okuda, J., Ishibashi, M., Iwanaga, M., Garg, P., Rammamurthy, T., Wong, H.-C., Depaola, A., Kim, Y. B., Albert, M. J., Nishibuchi, M. (2000). Pandemic Spread of an O3:K6 Clone of Vibrio parahaemolyticus and Emergence of Related Strains Evidenced by Arbitrarily Primed PCR and toxRS Sequence Analyses. J. Clin. Microbiol. 38: 578-585 [Abstract] [Full Text]  
  • Jiang, S. C., Louis, V., Choopun, N., Sharma, A., Huq, A., Colwell, R. R. (2000). Genetic Diversity of Vibrio cholerae in Chesapeake Bay Determined by Amplified Fragment Length Polymorphism Fingerprinting. Appl. Environ. Microbiol. 66: 140-147 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»