This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bauer, J.
Right arrow Articles by Larsen, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bauer, J.
Right arrow Articles by Larsen, B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 600-605, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Typing of Clinical Mycobacterium avium Complex Strains Cultured during a 2-Year Period in Denmark by Using IS1245

Jeanett Bauer,1,* Åse B. Andersen,1 Dorthe Askgaard,2 Sten B. Giese,3 and Birger Larsen4

Department of Mycobacteriology, Statens Serum Institut,1 Department of Infectious Medicine, Rigshospitalet,2 and Veterinary Laboratory,3 Copenhagen, and Department of Infectious Medicine, Marselisborg Hospital, Århus,4 Denmark

Received 13 July 1998/Returned for modification 13 October 1998/Accepted 7 December 1998

In the present study restriction fragment length polymorphism analyses with the recently described insertion sequence IS1245 as a probe was performed with clinical Mycobacterium avium complex strains cultured in Denmark during a 2-year period. The overall aim of the study was to disclose potential routes of transmission of these microorganisms. As a first step, the genetic diversity among isolates from AIDS patients and non-human immunodeficiency virus (HIV)-infected patients was described. In addition, a number of isolates from nonhuman sources cultured during the same period were analyzed and compared to the human isolates. A total of 203 isolates from AIDS patients (n = 90), non-HIV-infected patients (n = 91), and nonhuman sources (n = 22) were analyzed. The presence of IS1245 was restricted to Mycobacterium avium subsp. avium isolates. The majority of human isolates had large numbers of IS1245 copies, while nonhuman isolates could be divided into a high-copy-number group and a low-copy-number group. Groups of identical strains were found to be geographically widespread, comprising strains from AIDS patients as well as strains from non-HIV-infected patients. Samples of peat (to be used as potting soil) and veterinary samples were found to contain viable M. avium isolates belonging to genotypes also found in humans.

* Corresponding author. Mailing address: Department of Mycobacteriology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Phone: 45 3268 3705. Fax: 45 3268 3871. E-mail: jba{at}ssi.dk.

Journal of Clinical Microbiology, March 1999, p. 600-605, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Alvarez, J., Garcia, I. G., Aranaz, A., Bezos, J., Romero, B., de Juan, L., Mateos, A., Gomez-Mampaso, E., Dominguez, L. (2008). Genetic Diversity of Mycobacterium avium Isolates Recovered from Clinical Samples and from the Environment: Molecular Characterization for Diagnostic Purposes. J. Clin. Microbiol. 46: 1246-1251 [Abstract] [Full Text]  
  • Pfaller, S. L., Aronson, T. W., Holtzman, A. E., Covert, T. C. (2007). Amplified fragment length polymorphism analysis of Mycobacterium avium complex isolates recovered from southern California. J Med Microbiol 56: 1152-1160 [Abstract] [Full Text]  
  • Horan, K. L., Freeman, R., Weigel, K., Semret, M., Pfaller, S., Covert, T. C., van Soolingen, D., Leao, S. C., Behr, M. A., Cangelosi, G. A. (2006). Isolation of the Genome Sequence Strain Mycobacterium avium 104 from Multiple Patients over a 17-Year Period.. J. Clin. Microbiol. 44: 783-789 [Abstract] [Full Text]  
  • Johansen, T. B., Djonne, B., Jensen, M. R., Olsen, I. (2005). Distribution of IS1311 and IS1245 in Mycobacterium avium Subspecies Revisited. J. Clin. Microbiol. 43: 2500-2502 [Abstract] [Full Text]  
  • Oliveira, R. S., Sircili, M. P., Oliveira, E. M. D., Balian, S. C., Ferreira-Neto, J. S., Leao, S. C. (2003). Identification of Mycobacterium avium Genotypes with Distinctive Traits by Combination of IS1245-Based Restriction Fragment Length Polymorphism and Restriction Analysis of hsp65. J. Clin. Microbiol. 41: 44-49 [Abstract] [Full Text]  
  • Kunimoto, D. Y., Peppler, M. S., Talbot, J., Phillips, P., Shafran, S. D. (2003). Analysis of Mycobacterium avium Complex Isolates from Blood Samples of AIDS Patients by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 41: 498-499 [Abstract] [Full Text]  
  • Dvorska, L., Bartos, M., Ostadal, O., Kaustova, J., Matlova, L., Pavlik, I. (2002). IS1311 and IS1245 Restriction Fragment Length Polymorphism Analyses, Serotypes, and Drug Susceptibilities of Mycobacterium avium Complex Isolates Obtained from a Human Immunodeficiency Virus-Negative Patient. J. Clin. Microbiol. 40: 3712-3719 [Abstract] [Full Text]  
  • NOVI, C., RINDI, L., LARI, N., GARZELLI, C. (2000). Molecular typing of Mycobacterium avium isolates by sequencing of the 16S-23S rDNA internal transcribed spacer and comparison with IS1245-based fingerprinting. J Med Microbiol 49: 1091-1095 [Abstract] [Full Text]  
  • Beggs, M. L., Stevanova, R., Eisenach, K. D. (2000). Species Identification of Mycobacterium avium Complex Isolates by a Variety of Molecular Techniques. J. Clin. Microbiol. 38: 508-512 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»