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Journal of Clinical Microbiology, March 1999, p. 600-605, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Typing of Clinical Mycobacterium avium Complex Strains Cultured during a 2-Year Period in Denmark by Using IS1245

Jeanett Bauer,1,* Åse B. Andersen,1 Dorthe Askgaard,2 Sten B. Giese,3 and Birger Larsen4

Department of Mycobacteriology, Statens Serum Institut,1 Department of Infectious Medicine, Rigshospitalet,2 and Veterinary Laboratory,3 Copenhagen, and Department of Infectious Medicine, Marselisborg Hospital, Århus,4 Denmark

Received 13 July 1998/Returned for modification 13 October 1998/Accepted 7 December 1998

In the present study restriction fragment length polymorphism analyses with the recently described insertion sequence IS1245 as a probe was performed with clinical Mycobacterium avium complex strains cultured in Denmark during a 2-year period. The overall aim of the study was to disclose potential routes of transmission of these microorganisms. As a first step, the genetic diversity among isolates from AIDS patients and non-human immunodeficiency virus (HIV)-infected patients was described. In addition, a number of isolates from nonhuman sources cultured during the same period were analyzed and compared to the human isolates. A total of 203 isolates from AIDS patients (n = 90), non-HIV-infected patients (n = 91), and nonhuman sources (n = 22) were analyzed. The presence of IS1245 was restricted to Mycobacterium avium subsp. avium isolates. The majority of human isolates had large numbers of IS1245 copies, while nonhuman isolates could be divided into a high-copy-number group and a low-copy-number group. Groups of identical strains were found to be geographically widespread, comprising strains from AIDS patients as well as strains from non-HIV-infected patients. Samples of peat (to be used as potting soil) and veterinary samples were found to contain viable M. avium isolates belonging to genotypes also found in humans.

* Corresponding author. Mailing address: Department of Mycobacteriology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Phone: 45 3268 3705. Fax: 45 3268 3871. E-mail: jba{at}ssi.dk.

Journal of Clinical Microbiology, March 1999, p. 600-605, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.