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Journal of Clinical Microbiology, March 1999, p. 753-757, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Impact of Microbiology Practice on Cumulative Prevalence of Respiratory Tract Bacteria in Patients with Cystic Fibrosis

Michael R. Shreve,1,* Steven Butler,2 Haley J. Kaplowitz,2 Harvey R. Rabin,3 Dennis Stokes,4 Michael Light,5 Warren E. Regelmann1 for North American Scientific Advisory Group and Investigators for the Epidemiologic Study of Cystic Fibrosis

University of Minnesota School of Medicine, Minneapolis, Minnesota1; Genentech, Inc., South San Francisco,2 and University of California, San Diego, La Jolla,5 California; Health Sciences Center, University of Calgary, Calgary, Alberta, Canada3; and Vanderbilt University Medical Center, Nashville, Tennessee4

Received 14 September 1998/Accepted 17 November 1998

Investigators participating in the Epidemiologic Study of Cystic Fibrosis project began to collect microbiological, pulmonary, and nutritional data on cystic fibrosis (CF) patients at 180 North American sites in 1994. Part of this study was a survey undertaken in August 1995 to determine microbiology laboratory practices with regard to pulmonary specimens from CF patients. The survey included a section on test ordering, completed by a site clinician, and a section on test performance and reporting, completed by each site's clinical microbiology laboratory staff. Seventy-nine percent of the surveys were returned. There was intersite consistency of microbiology laboratory practices in most cases. The majority of sites follow most of the CF Foundation consensus conference recommendations. There were differences in the frequency at which specimens for culture were obtained, in the use of selective media for Staphylococcus aureus and Haemophilus influenzae, and in the use of a prolonged incubation for Burkholderia cepacia. These variations in practice contribute to prevalence differences among sites and may result in differences in clinical care.


* Corresponding author. Mailing address: Department of Pediatrics, Division of Pulmonary and Critical Care Medicine, University of Minnesota School of Medicine, Box 742, 420 Delaware St. S.E., Minneapolis, MN 55044. Phone: (612) 626-4440. Fax: (612) 624-0696. E-mail: shrev002{at}gold.tc.umn.edu.


Journal of Clinical Microbiology, March 1999, p. 753-757, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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