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Journal of Clinical Microbiology, March 1999, p. 762-765, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of CHROMagar Salmonella Medium and Hektoen Enteric Agar for Isolation of Salmonellae from Stool Samples

Olivier Gaillot,1,2,* Patrick Di Camillo,1 Patrick Berche,1,2 René Courcol,3 and Colette Savage3

Laboratoire de Bactériologie-Virologie, Hôpital Boucicaut,1 Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades,2 75730 Paris, and Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional et Universitaire, 59045 Lille,3 France

Received 1 October 1998/Returned for modification 20 October 1998/Accepted 9 November 1998

CHROMagar Salmonella (CAS), a new chromogenic medium, was retrospectively compared to Hektoen enteric agar (HEA) with 501 Salmonella stock isolates and was then prospectively compared to HEA for the detection and presumptive identification of Salmonella spp. with 508 stool samples before and after enrichment. All stock cultures (100%), including cultures of H2S-negative isolates, yielded typical mauve colonies on CAS, while 497 (99%) isolates produced typical lactose-negative, black-centered colonies on HEA. Following overnight incubation at 37°C, a total of 20 Salmonella strains were isolated from the 508 clinical samples. Sensitivities for primary plating and after enrichment were 95% (19 isolates) and 100% (20 isolates), respectively, for CAS and 80% (16 isolates) and 100% (20 isolates), respectively, for HEA. The specificity of CAS (88.9%) was significantly higher than that of HEA (78.5%; P < 0.0001). On the basis of its good sensitivity and specificity, CAS medium can be recommended for use for primary plating when human stool samples are screened for Salmonella spp.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades, 75730 Paris Cedex 15, France. Phone: (33) (1) 40 61 53 77. Fax: (33) (1) 40 61 55 92. E-mail: gaillot{at}necker.fr.

Journal of Clinical Microbiology, March 1999, p. 762-765, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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