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Journal of Clinical Microbiology, March 1999, p. 782-784, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Assessment of Use of the COBAS AMPLICOR System with BACTEC 12B Cultures for Rapid Detection of Frequently Identified Mycobacteria

B. Ninet,* P. Rohner, C. Metral, and R. Auckenthaler

Division of Infectious Diseases, Laboratory of Bacteriology, Hôpital Cantonal Universitaire, CH 1211 Geneva, Switzerland

Received 29 June 1998/Returned for modification 13 October 1998/Accepted 21 November 1998

The use of the COBAS AMPLICOR System (Roche Molecular Diagnostics, Basel, Switzerland), the only automated system for PCR testing, was evaluated for a rapid identification of mycobacteria with positive BACTEC 12B cultures. Two hundred ninety-six specimens with a growth index of >= 30 were analyzed for the presence of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare. Compared to traditional methods and provided that samples with PCR inhibition are retested at a 1:10 dilution, the sensitivity and specificity of the COBAS AMPLICOR System with BACTEC 12B cultures were 100 and 98%, respectively. The COBAS AMPLICOR method is rapid and reliable for identifying the most common mycobacteria in cultures.

* Corresponding author. Mailing address: Division of Infectious Diseases, Laboratory of Bacteriology, Hôpital Cantonal Universitaire, CH 1211 Geneva, Switzerland. Phone: 00 41 22 372 73 09. Fax: 00 41 22 372 73 04. E-mail: Beatrice.Ninet{at}hcuge.ch.

Journal of Clinical Microbiology, March 1999, p. 782-784, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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