This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ninet, B.
Right arrow Articles by Auckenthaler, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ninet, B.
Right arrow Articles by Auckenthaler, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 782-784, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Assessment of Use of the COBAS AMPLICOR System with BACTEC 12B Cultures for Rapid Detection of Frequently Identified Mycobacteria

B. Ninet,* P. Rohner, C. Metral, and R. Auckenthaler

Division of Infectious Diseases, Laboratory of Bacteriology, Hôpital Cantonal Universitaire, CH 1211 Geneva, Switzerland

Received 29 June 1998/Returned for modification 13 October 1998/Accepted 21 November 1998

The use of the COBAS AMPLICOR System (Roche Molecular Diagnostics, Basel, Switzerland), the only automated system for PCR testing, was evaluated for a rapid identification of mycobacteria with positive BACTEC 12B cultures. Two hundred ninety-six specimens with a growth index of >= 30 were analyzed for the presence of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare. Compared to traditional methods and provided that samples with PCR inhibition are retested at a 1:10 dilution, the sensitivity and specificity of the COBAS AMPLICOR System with BACTEC 12B cultures were 100 and 98%, respectively. The COBAS AMPLICOR method is rapid and reliable for identifying the most common mycobacteria in cultures.

* Corresponding author. Mailing address: Division of Infectious Diseases, Laboratory of Bacteriology, Hôpital Cantonal Universitaire, CH 1211 Geneva, Switzerland. Phone: 00 41 22 372 73 09. Fax: 00 41 22 372 73 04. E-mail: Beatrice.Ninet{at}hcuge.ch.

Journal of Clinical Microbiology, March 1999, p. 782-784, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lefmann, M., Moter, A., Schweickert, B., Gobel, U. B. (2005). Misidentification of Mycobacterium leprae as Mycobacterium intracellulare by the COBAS AMPLICOR M. intracellulare Test. J. Clin. Microbiol. 43: 1928-1929 [Abstract] [Full Text]  
  • Zheng, X., Pang, M., Engler, H. D., Tanaka, S., Reppun, T. (2001). Rapid Detection of Mycobacterium tuberculosis in Contaminated BACTEC 12B Broth Cultures by Testing with Amplified Mycobacterium Tuberculosis Direct Test. J. Clin. Microbiol. 39: 3718-3720 [Abstract] [Full Text]  
  • Desmond, E. P., Loretz, K. (2001). Use of the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for Early Detection of Mycobacterium tuberculosis in BACTEC 12B Medium. J. Clin. Microbiol. 39: 1993-1995 [Abstract] [Full Text]  
  • Katila, M.-L., Katila, P., Erkinjuntti-Pekkanen, R. (2000). Accelerated Detection and Identification of Mycobacteria with MGIT 960 and COBAS AMPLICOR Systems. J. Clin. Microbiol. 38: 960-964 [Abstract] [Full Text]  
  • Pfyffer, G. E., Bonato, D. A., Ebrahimzadeh, A., Gross, W., Hotaling, J., Kornblum, J., Laszlo, A., Roberts, G., Salfinger, M., Wittwer, F., Siddiqi, S. (1999). Multicenter Laboratory Validation of Susceptibility Testing of Mycobacterium tuberculosis against Classical Second-Line and Newer Antimicrobial Drugs by Using the Radiometric BACTEC 460 Technique and the Proportion Method with Solid Media. J. Clin. Microbiol. 37: 3179-3186 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»