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Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
The IS6110 Restriction Fragment Length
Polymorphism in Particular Multidrug-Resistant Mycobacterium
tuberculosis Strains May Evolve Too Fast for Reliable Use in
Outbreak Investigation
Alicia
Alito,1
Nora
Morcillo,2
Silvia
Scipioni,2
Alberto
Dolmann,2
María I.
Romano,3
Angel
Cataldi,3 and
Dick
van Soolingen4,*
Pathobiology Institute
CICV/INTA1 and
Biotechnology Institute
CICV/INTA,3 Morón, and
Dr.
Cetrángolo Hospital, Vte. López,2
Argentina, and
National Institute of Public Health and the
Environment, 3720 BA Bilthoven, The
Netherlands4
Received 22 June 1998/Returned for modification 18 August
1998/Accepted 3 November 1998
To study possible nosocomial transmission of multidrug-resistant
(MDR) Mycobacterium tuberculosis, strain types and other information on 24, mostly human immunodeficiency virus-positive patients, were collected. Isolates from 11 patients had identical IS6110 restriction fragment length polymorphism (RFLP)
patterns as well as spoligotype patterns and resistance profiles.
Noticeably, nine other isolates from related cases also exhibited
identical spoligotypes but slightly different RFLP patterns. These
results indicate that for some MDR strains, the evolutionary clock of IS6110 RFLP may run too fast for reliable interpretation of
strain typing results over a period of a few years.
*
Corresponding author. Mailing address: National
Institute of Public Health and the Environment, P.O. Box 1, 3720 BA
Bilthoven, The Netherlands. Phone: 31-30-2742363. Fax: 31-30-2744414. E-mail: d.van.soolingen{at}rivm.nl.
Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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