This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alito, A.
Right arrow Articles by van Soolingen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alito, A.
Right arrow Articles by van Soolingen, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The IS6110 Restriction Fragment Length Polymorphism in Particular Multidrug-Resistant Mycobacterium tuberculosis Strains May Evolve Too Fast for Reliable Use in Outbreak Investigation

Alicia Alito,1 Nora Morcillo,2 Silvia Scipioni,2 Alberto Dolmann,2 María I. Romano,3 Angel Cataldi,3 and Dick van Soolingen4,*

Pathobiology Institute CICV/INTA1 and Biotechnology Institute CICV/INTA,3 Morón, and Dr. Cetrángolo Hospital, Vte. López,2 Argentina, and National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands4

Received 22 June 1998/Returned for modification 18 August 1998/Accepted 3 November 1998

To study possible nosocomial transmission of multidrug-resistant (MDR) Mycobacterium tuberculosis, strain types and other information on 24, mostly human immunodeficiency virus-positive patients, were collected. Isolates from 11 patients had identical IS6110 restriction fragment length polymorphism (RFLP) patterns as well as spoligotype patterns and resistance profiles. Noticeably, nine other isolates from related cases also exhibited identical spoligotypes but slightly different RFLP patterns. These results indicate that for some MDR strains, the evolutionary clock of IS6110 RFLP may run too fast for reliable interpretation of strain typing results over a period of a few years.

* Corresponding author. Mailing address: National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31-30-2742363. Fax: 31-30-2744414. E-mail: d.van.soolingen{at}rivm.nl.

Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Borrell, S., Espanol, M., Orcau, A., Tudo, G., March, F., Cayla, J. A., Jansa, J. M., Alcaide, F., Martin-Casabona, N., Salvado, M., Martinez, J. A., Vidal, R., Sanchez, F., Altet, N., Coll, P., Gonzalez-Martin, J. (2009). Factors Associated with Differences between Conventional Contact Tracing and Molecular Epidemiology in Study of Tuberculosis Transmission and Analysis in the City of Barcelona, Spain. J. Clin. Microbiol. 47: 198-204 [Abstract] [Full Text]  
  • Gopaul, K. K., Brown, T. J., Gibson, A. L., Yates, M. D., Drobniewski, F. A. (2006). Progression Toward an Improved DNA Amplification-Based Typing Technique in the Study of Mycobacterium tuberculosis Epidemiology.. J. Clin. Microbiol. 44: 2492-2498 [Abstract] [Full Text]  
  • Allix, C., Walravens, K., Saegerman, C., Godfroid, J., Supply, P., Fauville-Dufaux, M. (2006). Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and Comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping.. J. Clin. Microbiol. 44: 1951-1962 [Abstract] [Full Text]  
  • Blackwood, K. S., Wolfe, J. N., Kabani, A. M. (2004). Application of Mycobacterial Interspersed Repetitive Unit Typing to Manitoba Tuberculosis Cases: Can Restriction Fragment Length Polymorphism Be Forgotten?. J. Clin. Microbiol. 42: 5001-5006 [Abstract] [Full Text]  
  • Otsuka, Y., Parniewski, P., Zwolska, Z., Kai, M., Fujino, T., Kirikae, F., Toyota, E., Kudo, K., Kuratsuji, T., Kirikae, T. (2004). Characterization of a Trinucleotide Repeat Sequence (CGG)5 and Potential Use in Restriction Fragment Length Polymorphism Typing of Mycobacterium tuberculosis. J. Clin. Microbiol. 42: 3538-3548 [Abstract] [Full Text]  
  • Erler, W., Martin, G., Sachse, K., Naumann, L., Kahlau, D., Beer, J., Bartos, M., Nagy, G., Cvetnic, Z., Zolnir-Dovc, M., Pavlik, I. (2004). Molecular Fingerprinting of Mycobacterium bovis subsp. caprae Isolates from Central Europe. J. Clin. Microbiol. 42: 2234-2238 [Abstract] [Full Text]  
  • Savine, E., Warren, R. M., van der Spuy, G. D., Beyers, N., van Helden, P. D., Locht, C., Supply, P. (2002). Stability of Variable-Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 4561-4566 [Abstract] [Full Text]  
  • Cameron, H., O'Brien, R., Murray, A., Cryan, B., Hone, R., Rogers, M. (2001). Evaluation of the Mycobacterium bovis Restriction Fragment Length Polymorphism Probe pUCD, in Combination with the Direct Repeat Probe, for Molecular Typing of Mycobacterium tuberculosis Strains in Ireland. J. Clin. Microbiol. 39: 4404-4406 [Abstract] [Full Text]  
  • Haddad, N., Ostyn, A., Karoui, C., Masselot, M., Thorel, M. F., Hughes, S. L., Inwald, J., Hewinson, R. G., Durand, B. (2001). Spoligotype Diversity of Mycobacterium bovis Strains Isolated in France from 1979 to 2000. J. Clin. Microbiol. 39: 3623-3632 [Abstract] [Full Text]  
  • CAMINERO, J. A., PENA, M. J., CAMPOS-HERRERO, M. I., RODRIGUEZ, J. C., GARCIA, I., CABRERA, P., LAFOZ, C., SAMPER, S., TAKIFF, H., AFONSO, O., PAVON, J. M., TORRES, M. J., SOOLINGEN, D. V., ENARSON, D. A., MARTIN, C. (2001). Epidemiological Evidence of the Spread of a Mycobacterium tuberculosis Strain of the Beijing Genotype on Gran Canaria Island. Am. J. Respir. Crit. Care Med. 164: 1165-1170 [Abstract] [Full Text]  
  • Kruuner, A., Hoffner, S. E., Sillastu, H., Danilovits, M., Levina, K., Svenson, S. B., Ghebremichael, S., Koivula, T., Kallenius, G. (2001). Spread of Drug-Resistant Pulmonary Tuberculosis in Estonia. J. Clin. Microbiol. 39: 3339-3345 [Abstract] [Full Text]  
  • Barlow, R. E. L., Gascoyne-Binzi, D. M., Gillespie, S. H., Dickens, A., Qamer, S., Hawkey, P. M. (2001). Comparison of Variable Number Tandem Repeat and IS6110-Restriction Fragment Length Polymorphism Analyses for Discrimination of High- and Low-Copy-Number IS6110 Mycobacterium tuberculosis Isolates. J. Clin. Microbiol. 39: 2453-2457 [Abstract] [Full Text]  
  • Mazars, E., Lesjean, S., Banuls, A.-L., Gilbert, M., Vincent, V., Gicquel, B., Tibayrenc, M., Locht, C., Supply, P. (2001). High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology. Proc. Natl. Acad. Sci. USA 98: 1901-1906 [Abstract] [Full Text]  
  • Niemann, S., Rüsch-Gerdes, S., Richter, E., Thielen, H., Heykes-Uden, H., Diel, R. (2000). Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosis Strains in Actual Chains of Transmission. J. Clin. Microbiol. 38: 2563-2567 [Abstract] [Full Text]  
  • Millemann, Y., Gaubert, S., Remy, D., Colmin, C. (2000). Evaluation of IS200-PCR and Comparison with Other Molecular Markers To Trace Salmonella enterica subsp. enterica Serotype Typhimurium Bovine Isolates from Farm to Meat. J. Clin. Microbiol. 38: 2204-2209 [Abstract] [Full Text]  
  • Filliol, I., Sola, C., Rastogi, N. (2000). Detection of a Previously Unamplified Spacer within the DR Locus of Mycobacterium tuberculosis: Epidemiological Implications. J. Clin. Microbiol. 38: 1231-1234 [Abstract] [Full Text]  
  • Niemann, S., Richter, E., Rüsch-Gerdes;, S., van Soolingen, D., de Boer, A. S., Alito, A., Morcillo, N. (1999). Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Multidrug-Resistant Mycobacterium tuberculosis Strains. J. Clin. Microbiol. 37: 3078-3079 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»