The IS6110 Restriction Fragment Length Polymorphism in Particular Multidrug-Resistant Mycobacterium tuberculosis Strains May Evolve Too Fast for Reliable Use in Outbreak Investigation

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Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The IS6110 Restriction Fragment Length Polymorphism in Particular Multidrug-Resistant Mycobacterium tuberculosis Strains May Evolve Too Fast for Reliable Use in Outbreak Investigation

Alicia Alito,¹ Nora Morcillo,² Silvia Scipioni,² Alberto Dolmann,² María I. Romano,³ Angel Cataldi,³ and Dick van Soolingen⁴^,^*

Pathobiology Institute CICV/INTA¹ and Biotechnology Institute CICV/INTA,³ Morón, and Dr. Cetrángolo Hospital, Vte. López,² Argentina, and National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands⁴

Received 22 June 1998/Returned for modification 18 August 1998/Accepted 3 November 1998

To study possible nosocomial transmission of multidrug-resistant (MDR) Mycobacterium tuberculosis, strain types and otherinformation on 24, mostly human immunodeficiency virus-positivepatients, were collected. Isolates from 11 patients had identicalIS6110 restriction fragment length polymorphism (RFLP) patternsas well as spoligotype patterns and resistance profiles. Noticeably,nine other isolates from related cases also exhibited identicalspoligotypes but slightly different RFLP patterns. These resultsindicate that for some MDR strains, the evolutionary clock ofIS6110 RFLP may run too fast for reliable interpretation of straintyping results over a period of a fewyears.

^* Corresponding author. Mailing address: National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven,The Netherlands. Phone: 31-30-2742363. Fax: 31-30-2744414. E-mail:d.van.soolingen{at}rivm.nl.

Journal of Clinical Microbiology, March 1999, p. 788-791, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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