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Journal of Clinical Microbiology, March 1999, p. 796-797, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparative Evaluation of First- and Second-Generation Digene Hybrid Capture Assays for Detection of Human Papillomaviruses Associated with High or Intermediate Risk for Cervical Cancer

Mario Poljak,1,* Anja Brencic,1 Katja Seme,1 Adriana Vince,2 and Irena J. Marin1

Institute of Microbiology and Immunology, Medical Faculty, Ljubljana, Slovenia,1 and Dr. Fran Mihaljevic' University Hospital for Infectious Diseases, Zagreb, Croatia2

Received 2 July 1998/Returned for modification 22 October 1998/Accepted 17 November 1998

In the present study we comparatively evaluated the first- and second-generation Digene Hybrid Capture assays for detection of human papillomaviruses (HPV) associated with high or intermediate risk for cervical cancer in cervical specimens. Concordant results were obtained with 468 of 483 (96.8%) specimens. All 15 specimens which gave repeatedly discordant results were positive by the second-generation test only, and 14 of them tested PCR positive. The enhanced sensitivity of the second-generation assay is mainly a result of the reformulation of hybridization reagents and, to a lesser extent, a result of the addition of new HPV probes.

* Corresponding author. Mailing address: Institute of Microbiology and Immunology, Medical Faculty, Zaloska 4, 1105 Ljubljana, Slovenia. Phone: 386 61 1403 042. Fax: 386 61 1423 518. E-mail: poljak{at}animus.mf.uni-lj.si.

Journal of Clinical Microbiology, March 1999, p. 796-797, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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