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Journal of Clinical Microbiology, March 1999, p. 815-817, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Simple and Rapid Methods for Identifying Enterococci Intrinsically Resistant to Vancomycin

Kevan L. Hanson1 and Charles P. Cartwright1,2,*

Microbiology Department, Clinical Laboratories, Hennepin County Medical Center, Minneapolis, Minnesota 55415,1 and Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 554552

Received 24 August 1998/Returned for modification 7 October 1998/Accepted 4 December 1998

Three different methodologies, reduction of litmus milk (LM) and acidification of arabinose (ARA), acidification of methyl-alpha -D-glucopyranoside (MGP), and rapid motility (RM), for differentiating isolates of Enterococcus casseliflavus and Enterococcus gallinarum (intrinsically vancomycin-resistant enterococci [IVRE]) from Enterococcus faecalis and Enterococcus faecium were evaluated. All 33 isolates of E. faecalis tested reduced LM within 4 h and were negative in all other tests, while the 53 isolates of E. faecium were ARA positive only. In contrast, 45 of 46 (98%) IVRE isolates examined (26 E. casseliflavus and 20 E. gallinarum isolates) acidified MGP, 41 of 46 (89%) were LM and ARA positive, and 45 of 46 (98%) were RM positive. Acidification of MGP was therefore the single most useful test for differentiating IVRE from vancomycin-resistant E. faecium and E. faecalis; however, a combination of LM-ARA and RM testing enabled the correct designation of organisms without the need for overnight incubation.

* Corresponding author. Mailing address: Clinical Laboratories, MC #812, Hennepin County Medical Center, 701 Park Ave., Minneapolis, MN 55415. Phone: (612) 347-3026. Fax: (612) 904-4229. E-mail: cartw006{at}tc.umn.edu.

Journal of Clinical Microbiology, March 1999, p. 815-817, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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