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Journal of Clinical Microbiology, March 1999, p. 830-831, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Contaminating Fungal DNA Sequences in Zymolyase

Dagmar Rimek,1,* Amar P. Garg,2 Walter H. Haas,3 and Reinhard Kappe1

Hygiene Institute1 and Department of General Pediatrics, Children's Hospital,3 University of Heidelberg, Heidelberg, Germany, and Department of Botany, C. Charan Singh University, Meerut, India2

Received 27 August 1998/Returned for modification 22 October 1998/Accepted 11 December 1998

When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.

* Corresponding author. Mailing address: Hygiene-Institut, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany. Phone: 49 6221 567816. Fax: 49 6221 564343. E-mail: dagmar_rimek{at}ukl.uni-heidelberg.de.

Journal of Clinical Microbiology, March 1999, p. 830-831, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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