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Journal of Clinical Microbiology, March 1999, p. 832-834, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Optimal Detection and Identification of Mycobacterium haemophilum in Specimens from Pediatric Patients with Cervical Lymphadenopathy

Zmira Samra,1,* Lea Kaufmann,1 Avraham Zeharia,2 Shai Ashkenazi,2 Jacob Amir,2 Judy Bahar,3 Udo Reischl,4 and Ludmilla Naumann4

Department of Microbiology, Rabin Medical Center,1 and Schneider Children's Medical Center of Israel,2 Petah Tiqva, and Hy-Laboratories, Ltd., Rehovot,3 Israel, and Institute for Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany4

Received 24 August 1998/Returned for modification 12 October 1998/Accepted 30 November 1998

Acid-fast bacilli from pediatric patients with lymphadenopathy were detected in the BACTEC radiometric system and in MB Redox broth, but not on Löwenstein Jensen medium. PCR amplification identified the isolates as Mycobacterium haemophilum, which has special nutrition requirements (iron supplements) for growth. Suitable culture medium ensures optimal recovery of this microorganism, avoiding underdiagnosis.

* Corresponding author. Mailing address: Laboratory of Clinical Microbiology, Rabin Medical Center, Beilinson Campus, Petah Tiqva 49100, Israel. Phone: 972-3-937 6725. Fax: 972-3-921 8466. E-mail: HY-LABS{at}Internet.Zahav.net.

Journal of Clinical Microbiology, March 1999, p. 832-834, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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