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Journal of Clinical Microbiology, March 1999, p. 867-869, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Seven Treponema Species in Health- and Disease-Associated Dental Plaque by Nested PCR

S. G. Willis,1 K. S. Smith,1 V. L. Dunn,2 L. A. Gapter,1,dagger K. H. Riviere,1 and G. R. Riviere1,*

Departments of Pediatric Dentistry1 and Periodontology,2 Oregon Health Sciences University, Portland, Oregon 97201-3097

Received 3 August 1998/Returned for modification 28 September 1998/Accepted 18 November 1998

Species-specific nested PCR was used to detect Treponema amylovorum, Treponema denticola, Treponema maltophilum, Treponema medium, Treponema pectinovorum, Treponema socranskii, and Treponema vincentii in dental plaque. Subjects with periodontitis harbored all species, but T. pectinovorum and T. vincentii were not found in plaque from disease-free subjects.


* Corresponding author. Mailing address: OHSU School of Dentistry SD182, 611 SW Campus Dr., Portland, OR 97201-3097. Phone: (503) 494-8489. Fax: (503) 494-4666. E-mail: riviereg{at}ohsu.edu.

dagger Present address: Department of Genetics and Cell Biology, Washington State University, Pullman, WA 99164-4234.


Journal of Clinical Microbiology, March 1999, p. 867-869, Vol. 37, No. 3
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



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