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Journal of Clinical Microbiology, April 1999, p. 1024-1029, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Indirect Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Reactive with a Recombinant Protein Expressed from the Gene Encoding the 116-Kilodalton Protein of Mycoplasma pneumoniae

Michael F. Duffy,1,dagger Kevin G. Whithear,1 Amir H. Noormohammadi,1 Philip F. Markham,1,* Michael Catton,2 Jennie Leydon,2 and Glenn F. Browning1

Department of Veterinary Science, The University of Melbourne,1 and Victorian Infectious Diseases Reference Laboratory,2 Parkville, Victoria 3052, Australia

Received 1 June 1998/Returned for modification 4 August 1998/Accepted 7 January 1999

Serology remains the method of choice for laboratory diagnosis of Mycoplasma pneumoniae infection. Currently available serological tests employ complex cellular fractions of M. pneumoniae as antigen. To improve the specificity of M. pneumoniae diagnosis, a recombinant protein was assessed as a serodiagnostic reagent. A panel of recombinant proteins were expressed from a cloned M. pneumoniae gene that encodes a 116-kDa surface protein antigen. The recombinant proteins were assessed for reactivity with patient sera and the most antigenic was further assessed for its serodiagnostic potential by indirect enzyme-linked immunosorbent assay (ELISA). The ELISA based on the recombinant protein was equivalent in sensitivity to the commercial test (Serodia Myco II; Fujirebio Inc.) to which it was compared. Southern and Western blotting data suggested that the recombinant protein derived from the 116-kDa protein of M. pneumoniae could provide a species-specific diagnostic tool, although further assessment is required.

* Corresponding author. Mailing address: Department of Veterinary Science, The University of Melbourne, Parkville, Victoria 3052, Australia. Phone: 61 3 93447368. Fax: 61 3 9344 7374. E-mail: p.markham{at}vet.unimelb.edu.au.

dagger Present address: Walter and Elisa Hall Institute for Medical Research, Parkville, Victoria 3052, Australia.

Journal of Clinical Microbiology, April 1999, p. 1024-1029, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.