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Journal of Clinical Microbiology, April 1999, p. 1062-1068, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis with a Combination of Macrorestriction Endonucleases Reveals a High Degree of Polymorphism among Bordetella pertussis Isolates in Eastern France

G. Prevost,1 F. I. S. Freitas,1 P. Stoessel,1 O. Meunier,2 M. Haubensack,1 H. Monteil,1 and J. M. Scheftel1,*

Institut de Bactériologie de la Faculté de Médecine de Strasbourg1 and Institut d'Hygiène de la Faculté de Médecine de Strasbourg,2 Université Louis Pasteur-Hôpitaux Universitaires de Strasbourg, F-67000 Strasbourg, France

Received 23 March 1998/Returned for modification 4 April 1998/Accepted 29 December 1998

From 1990 to 1996, routine screening for whooping cough identified 399 patients with a calmodulin-dependent adenylate cyclase-positive test result and yielded 69 Bordetella pertussis isolates. None of the patients were fully vaccinated, and most were less than 6 months old. Analysis of total DNA by pulsed-field gel electrophoresis (PFGE) after XbaI, SpeI, or DraI macrorestriction yielded 19, 15, and 5 different patterns, respectively, whereas ribotyping failed to demonstrate any strain polymorphism. Discrimination among the isolates was improved by combining the PFGE profiles. Some patterns were more frequent, but the corresponding patients were not clearly epidemiologically related. The patterns for two strains obtained during a 3-month period from patients who were neighbors differed by the length of a single DNA fragment. These data strongly suggest that one type of isolate is widely spread throughout the world and is carried by individuals other than patients who develop a true illness.

* Corresponding author. Mailing address: Institut de Bactériologie de la Faculté de Médecine de Strasbourg, Université Louis Pasteur-Hôpitaux Universitaires, 3, rue Koeberlé, F-67000 Strasbourg, France. Phone: (33) 3 88 21 19 70. Fax: (33) 3 88 25 11 13. E-mail: jmichel.scheftel{at}medecine.u_strasbg.fr.

Journal of Clinical Microbiology, April 1999, p. 1062-1068, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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