This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Crawford-Miksza, L. K.
Right arrow Articles by Schnurr, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crawford-Miksza, L. K.
Right arrow Articles by Schnurr, D. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1999, p. 1107-1112, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Strain Variation in Adenovirus Serotypes 4 and 7a Causing Acute Respiratory Disease

Leta K. Crawford-Miksza,1,* Roberto N. Nang,2 and David P. Schnurr1

Viral and Rickettsial Disease Laboratory, Division of Communicable Disease Control, California State Department of Health Services, Berkeley, California 94704,1 and U.S. Army Center for Health Promotion and Preventive Medicine, Aberdeen Proving Ground, Maryland 210102

Received 5 August 1998/Returned for modification 30 September 1998/Accepted 18 November 1998

In order to determine the suitability of vaccine strains established in the 1960s for a new vaccine, a comprehensive study of strain variation of adenovirus serotype 4 (AV 4) and AV 7 was undertaken. A 1,500-bp region of the hexon gene containing the AV neutralization epitopes from prototype, vaccine, and community-acquired strains and from wild-type strains from military personnel that cause acute respiratory disease (ARD) was sequenced and analyzed. The whole hexon gene from prototype strains, vaccine strains, and selected isolates was sequenced. AV 7 and AV 7a were found to have distinct genotypes, and all vaccine and wild-type strains recovered from 1963 to 1997 had the AV 7a genotype. There was no significant strain variation in the neutralization epitopes of the AV 7a genotype over a 42-year period. The evolution of AV 4 was more complex, with continuous genetic drift punctuated by replacement with a new strain. The current strain of AV 4, which has been in circulation since 1995, is significantly different from the AV 4 prototype and the vaccine strains. Genetic differences were confirmed to be antigenic differences by neutralization tests, which define the new strain as an AV 4 variant. A type-specific PCR for AV 4, AV 7/7a, and AV 21 was developed, and this PCR facilitated the rapid identification of isolates from outbreaks of ARD.

* Corresponding author. Mailing address: Viral and Rickettsial Disease Laboratory, California Department of Health Services, 2151 Berkeley Way, Berkeley CA 94704. Phone: (510) 540-2560. Fax: (510) 540-3305.

Journal of Clinical Microbiology, April 1999, p. 1107-1112, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ebner, K., Rauch, M., Preuner, S., Lion, T. (2006). Typing of Human Adenoviruses in Specimens from Immunosuppressed Patients by PCR-Fragment Length Analysis and Real-Time Quantitative PCR. J. Clin. Microbiol. 44: 2808-2815 [Abstract] [Full Text]  
  • Madisch, I., Harste, G., Pommer, H., Heim, A. (2005). Phylogenetic Analysis of the Main Neutralization and Hemagglutination Determinants of All Human Adenovirus Prototypes as a Basis for Molecular Classification and Taxonomy. J. Virol. 79: 15265-15276 [Abstract] [Full Text]  
  • Ebner, K., Pinsker, W., Lion, T. (2005). Comparative Sequence Analysis of the Hexon Gene in the Entire Spectrum of Human Adenovirus Serotypes: Phylogenetic, Taxonomic, and Clinical Implications. J. Virol. 79: 12635-12642 [Abstract] [Full Text]  
  • Purkayastha, A., Su, J., McGraw, J., Ditty, S. E., Hadfield, T. L., Seto, J., Russell, K. L., Tibbetts, C., Seto, D. (2005). Genomic and Bioinformatics Analyses of HAdV-4vac and HAdV-7vac, Two Human Adenovirus (HAdV) Strains That Constituted Original Prophylaxis against HAdV-Related Acute Respiratory Disease, a Reemerging Epidemic Disease. J. Clin. Microbiol. 43: 3083-3094 [Abstract] [Full Text]  
  • Krafft, A. E., Russell, K. L., Hawksworth, A. W., McCall, S., Irvine, M., Daum, L. T., Connoly, J. L., Reid, A. H., Gaydos, J. C., Taubenberger, J. K. (2005). Evaluation of PCR Testing of Ethanol-Fixed Nasal Swab Specimens as an Augmented Surveillance Strategy for Influenza Virus and Adenovirus Identification. J. Clin. Microbiol. 43: 1768-1775 [Abstract] [Full Text]  
  • Purkayastha, A., Ditty, S. E., Su, J., McGraw, J., Hadfield, T. L., Tibbetts, C., Seto, D. (2005). Genomic and Bioinformatics Analysis of HAdV-4, a Human Adenovirus Causing Acute Respiratory Disease: Implications for Gene Therapy and Vaccine Vector Development. J. Virol. 79: 2559-2572 [Abstract] [Full Text]  
  • Jacobs, S. C., Davison, A. J., Carr, S., Bennett, A. M., Phillpotts, R., Wilkinson, G. W. G. (2004). Characterization and manipulation of the human adenovirus 4 genome. J. Gen. Virol. 85: 3361-3366 [Abstract] [Full Text]  
  • Blasiole, D. A., Metzgar, D., Daum, L. T., Ryan, M. A. K., Wu, J., Wills, C., Le, C. T., Freed, N. E., Hansen, C. J., Gray, G. C., Russell, K. L. (2004). Molecular Analysis of Adenovirus Isolates from Vaccinated and Unvaccinated Young Adults. J. Clin. Microbiol. 42: 1686-1693 [Abstract] [Full Text]  
  • Xu, W., McDonough, M. C., Erdman, D. D. (2000). Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay. J. Clin. Microbiol. 38: 4114-4120 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»