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Journal of Clinical Microbiology, April 1999, p. 1117-1122, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Bartonella koehlerae sp. nov., Isolated
from Cats
Sara
Droz,1,2,*
Banghee
Chi,2
Elke
Horn,2
Arnold G.
Steigerwalt,3
Anne M.
Whitney,3 and
Don J.
Brenner3
Institute for Medical Microbiology,
University of Berne, CH-3010 Berne,
Switzerland1; Division of Infectious
Diseases, Department of Medicine, University of California, San
Francisco, California 94143-06542; and
Meningitis and Special Pathogens Branch, Division of
Bacterial and Mycotic Diseases, National Center for Infectious
Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia
303333
Received 2 July 1998/Returned for modification 22 September
1998/Accepted 11 January 1999
Two of the 25 Bartonella isolates recovered during a
prevalence study of Bartonella henselae bacteremia in
domestic cats from the greater San Francisco Bay region were found to
differ phenotypically and genotypically from all prior B. henselae isolates. These isolates, C-29 and C-30, which were
recovered from the blood of two pet cats belonging to the same
household, grew on chocolate agar as pinpoint colonies following 14 days of incubation at 35°C in a candle jar but failed to grow on
heart infusion agar supplemented with 5% rabbit blood. Additional
phenotypic characteristics distinguished the isolates C-29 and C-30
from other feline B. henselae isolates. The
restriction patterns obtained for C-29 and C-30 by citrate synthase
PCR-restriction fragment length polymorphism (RFLP) analysis as well as
by genomic RFLP could not be distinguished from each other but were
distinctly different from that of the B. henselae type
strain. In reciprocal reactions, DNAs from strains C-29 and C-30 were
97 to 100% related under optimal and stringent DNA reassociation conditions, with 0 to 0.5% divergence within related sequences. Labeled DNA from the type strain of B. henselae was 61 to 65% related to unlabeled DNAs from strains C-29 and C-30 in 55°C
reactions, with 5.0 to 5.5% divergence within the related sequences,
and 31 to 41% related in stringent, 70°C reactions. In reciprocal reactions, labeled DNAs from strains C-29 and C-30 were 68 to 92%
related to those of the B. henselae type strain and
other B. henselae strains, with 5 to 7% divergence.
The 16S rRNA gene sequence of strain C-29 was 99.54% homologous to
that of the type strain of B. henselae. On the basis
of these findings, the two isolates C-29 and C-30 are designated a new
species of Bartonella, for which we propose the name
Bartonella koehlerae. The type strain of Bartonella
koehlerae is strain C-29 (ATCC 700693).
*
Corresponding author. Mailing address: Institute for
Medical Microbiology, University of Berne, Friedbühlstrasse 51, CH-3010 Berne, Switzerland. Phone: 41 (31) 632-3265. Fax: 41 (31)
632-4965. E-mail: sara.droz{at}imm.unibe.ch.
Journal of Clinical Microbiology, April 1999, p. 1117-1122, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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