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Journal of Clinical Microbiology, April 1999, p. 1173-1177, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Vibrio parahaemolyticus Strains at the Species Level by PCR Targeted to the toxR Gene

Yung Bu Kim,^1, Jun Okuda,¹ Chiho Matsumoto,¹ Naoki Takahashi,² Satoru Hashimoto,² and Mitsuaki Nishibuchi^1,*

Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto,¹ and Kansai Airport Quarantine Station, Tajiri, Sennan, Osaka,² Japan

Received 10 August 1998/Returned for modification 14 October 1998/Accepted 21 December 1998

The DNA colony hybridization test with the polynucleotide probe for Vibrio parahaemolyticus toxR gene was performed. All 373 strains of V. parahaemolyticus gave positive results, and the strains belonging to four other Vibrio species including Vibrio alginolyticus gave weakly positive results, suggesting that toxR sequence variation may reflect the phylogenetic relationships of Vibrio species. We then established a toxR-targeted PCR protocol for the specific detection of V. parahaemolyticus.

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^* Corresponding author. Mailing address: Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-7367. Fax: 81-75-753-7350. E-mail: nishibuc{at}med.kyoto-u.ac.jp.

Present address: Department of Microbiology, College of Medicine, Pusan National University, Pusan 602-739, Korea.

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Journal of Clinical Microbiology, April 1999, p. 1173-1177, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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