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Journal of Clinical Microbiology, April 1999, p. 1197-1199, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

rpoB Mutations in Multidrug-Resistant Strains of Mycobacterium tuberculosis Isolated in Italy

G. Pozzi,1,* M. Meloni,2 E. Iona,3 G. Orrù,1 O. F. Thoresen,3 M. L. Ricci,3 M. R. Oggioni,1 L. Fattorini,3 and G. Orefici3

Sezione di Microbiologia, Dipartimento di Biologia Molecolare, Università di Siena, Siena,1 Sezione di Microbiologia, Dipartimento di Scienze Chirurgiche, Università di Cagliari, Cagliari,2 and Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, 00161 Rome,3 Italy

Received 6 August 1998/Returned for modification 21 October 1998/Accepted 21 December 1998

Mutations of rpoB associated with rifampin resistance were studied in 37 multidrug-resistant (MDR) clinical strains of Mycobacterium tuberculosis isolated in Italy. At least one mutated codon was found in each MDR strain. It was always a single-base substitution leading to an amino acid change. Nine different rpoB alleles, three of which had not been reported before, were found. The relative frequencies of specific mutations in this sample were different from those previously reported from different geographical areas, since 22 strains (59.5%) carried the mutated codon TTG in position 531 (Serright-arrowLeu) and 11 (29.7%) had GAC in position 526 (Hisright-arrowAsp).

* Corresponding author. Mailing address: Microbiologia/Università di Siena, Via Laterina 8, 53100 Siena, Italy. Phone: 39-0577-263874. Fax: 39-0577-263870. E-mail: pozzi{at}unisi.it.

Journal of Clinical Microbiology, April 1999, p. 1197-1199, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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