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Journal of Clinical Microbiology, April 1999, p. 1206-1209, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of the MB/BacT and BACTEC 460 TB Systems for Recovery of Mycobacteria from Various Clinical Specimens

Francesca Brunello,1 Flavio Favari,1 and Roberta Fontana1,2,*

Servizio di Microbiologia dell' Azienda Ospedaliera di Verona,1 and Istituto di Microbiologia dell'Università,2 Verona, Italy

Received 27 July 1998/Returned for modification 2 September 1998/Accepted 6 January 1999

A total of 1,830 specimens (75.7% respiratory and 24.3% nonrespiratory) were cultured in parallel with the MB/BacT and BACTEC 460 TB systems and on Lowenstein-Jensen (LJ) medium. Mycobacteria were identified from 173 (6.5%) specimens. The most common species recovered were Mycobacterium tuberculosis complex (65.9%), Mycobacterium avium complex (22.5%), and Mycobacterium chelonae (9.2%). The recovery rates by individual systems were 96.5, 99.4, and 95.9% for MB/BacT, BACTEC 460 TB, and LJ medium, respectively, for all mycobacteria; the recovery rates were 99.1, 100, and 98.2%, respectively, for M. tuberculosis complex alone. The difference among the recovery rates for all mycobacteria and those for individual species was not significant. The BACTEC 460 TB system detected M. tuberculosis isolates more rapidly than the MB/BacT system (8 versus 11.8 days for smear-positive specimens [P < 0.01] and 18 versus 21 days for smear-negative specimens [P < 0.05]), whereas the MB/BacT system more rapidly detected the nontuberculous mycobacteria (17.1 versus 12.7 days [P < 0.01]). These results indicate that the nonradiometric MB/BacT system is a rapid, sensitive, and efficient method for the recovery of M. tuberculosis and nontuberculous mycobacteria from both pulmonary and extrapulmonary clinical specimens.

* Corresponding author. Mailing address: Università di Verona, Istituto di Microbiologia, Strada Le Grazie 8, 37100 Verona, Italy. Phone: 0039-45-8098191. Fax: 0039-45-584606. E-mail: GIUSEPPE{at}BORGOROMA.UNIVR.IT.

Journal of Clinical Microbiology, April 1999, p. 1206-1209, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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