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Journal of Clinical Microbiology, April 1999, p. 920-924, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Phylogenetic Classification and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 Regions

Koichi Makimura,1,* Yoshiko Tamura,1 Takashi Mochizuki,2 Atsuhiko Hasegawa,3 Yoshito Tajiri,1 Ryo Hanazawa,1 Katsuhisa Uchida,1 Hiuga Saito,1 and Hideyo Yamaguchi1

Teikyo University Institute of Medical Mycology, Tokyo,1 Department of Dermatology, Kanazawa Medical University, Ishikawa,2 and Department of Veterinary Medicine, Nihon University, Kanagawa,3 Japan

Received 4 September 1998/Returned for modification 5 November 1998/Accepted 24 December 1998

The mutual phylogenetic relationships of dermatophytes of the genera Trichophyton, Microsporum, and Epidermophyton were demonstrated by using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences. Trichophyton spp. and Microsporum spp. form a cluster in the phylogenetic tree with Epidermophyton floccosum as an outgroup, and within this cluster, all Trichophyton spp. except Trichophyton terrestre form a nested cluster (100% bootstrap support). Members of dermatophytes in the cluster of Trichophyton spp. were classified into three groups with ITS1 homologies, with each of them being a monophyletic cluster (100% bootstrap support). The Arthroderma vanbreuseghemii-Arthroderma simii group consists of A. vanbreuseghemii, A. simii, Trichophyton mentagrophytes isolates from humans, T. mentagrophytes var. quinckeanum, Trichophyton tonsurans, and Trichophyton schoenleinii. Arthroderma benhamiae, T. mentagrophytes var. erinacei, and Trichophyton verrucosum are members of the Arthroderma benhamiae group. Trichophyton rubrum and Trichophyton violaceum form the T. rubrum group. This suggests that these "species" of dermatophytes have been overclassified. The ITS1 sequences of 11 clinical isolates were also determined to identify the species, and all strains were successfully identified by comparison of their base sequences with those in the ITS1 DNA sequence database.

* Corresponding author. Mailing address: Teikyo University Institute of Medical Mycology, Otsuka, Hachioji, Tokyo 192-0395 Japan. Phone: 81-426-78-3256. Fax: 81-426-74-9190. E-mail: makimura{at}main.teikyo-u.ac.jp.

Journal of Clinical Microbiology, April 1999, p. 920-924, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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