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Journal of Clinical Microbiology, April 1999, p. 954-957, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Nonlipophilic Corynebacteria Isolated from Dairy Cows with Mastitis

Jozef Hommez,1,* Luc A. Devriese,2 Mario Vaneechoutte,3 Philippe Riegel,4 Patrick Butaye,2 and Freddy Haesebrouck2

Regional Veterinary Laboratory, Torhout,1 Faculty of Veterinary Medicine, University of Ghent, Merelbeke,2 and Department of Chemistry, Microbiology and Immunology, University Hospital, Ghent,3 and Laboratoire de Bactériologie, Faculté de Médecine, Hôpitaux Universitaires, Strassbourg, France4

Received 2 July 1998/Returned for modification 22 September 1998/Accepted 17 December 1998

Nonlipophilic corynebacteria associated with clinical and subclinical mastitis in dairy cows were found to belong to four species: Corynebacterium amycolatum, Corynebacterium ulcerans, Corynebacterium pseudotuberculosis, and Corynebacterium minutissimum. These species may easily be confused. However, clear-cut differences between C. ulcerans and C. pseudotuberculosis were found in their acid production from maltotriose and ethylene glycol, susceptibility to vibriostatic agent O129, and alkaline phosphatase. Absence of growth at 20°C and lack of alpha -glucosidase and 4MU-alpha -D-glycoside hydrolysis activity differentiated C. amycolatum from C. pseudotuberculosis and C. ulcerans. The mastitis C. pseudotuberculosis strains differed from the biovar equi and ovis reference strains and from caprine field strains in their colony morphologies and in their reduced inhibitory activity on staphylococcal beta -hemolysin. C. amycolatum was the most frequently isolated nonlipophilic corynebacterium.

* Corresponding author. Mailing address: Regional Veterinary Laboratory, Industrielaan 15, B-8820 Torhout, Belgium. Phone: 32 50 230556. Fax: 32 50 230569.

Journal of Clinical Microbiology, April 1999, p. 954-957, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • De Zoysa, A., Hawkey, P. M., Engler, K., George, R., Mann, G., Reilly, W., Taylor, D., Efstratiou, A. (2005). Characterization of Toxigenic Corynebacterium ulcerans Strains Isolated from Humans and Domestic Cats in the United Kingdom. J. Clin. Microbiol. 43: 4377-4381 [Abstract] [Full Text]  
  • Devriese, L. A., Riegel, P., Hommez, J., Vaneechoutte, M., de Baere, T., Haesebrouck, F. (2000). Identification of Corynebacterium glucuronolyticum Strains from the Urogenital Tract of Humans and Pigs. J. Clin. Microbiol. 38: 4657-4659 [Abstract] [Full Text]  


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