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Journal of Clinical Microbiology, April 1999, p. 981-986, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Cell Wall Mannoprotein Mp1p in Culture Supernatants of Penicillium marneffei and in Sera of Penicilliosis Patients

Liang Cao,1,* King-Man Chan,1 Daliang Chen,1 Nongnuch Vanittanakom,2 Cindy Lee,1 Che-Man Chan,1 Thira Sirisanthana,3 Dominic N. C. Tsang,4 and Kwok-Yung Yuen1

Department of Microbiology, The University of Hong Kong,1 and Department of Pathology, Queen Elizabeth Hospital,4 Hong Kong, and Departments of Microbiology2 and Medicine,3 Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Received 9 September 1998/Returned for modification 7 December 1998/Accepted 24 December 1998

Mannoproteins are important and abundant structural components of fungal cell walls. The MP1 gene encodes a cell wall mannoprotein of the pathogenic fungus Penicillium marneffei. In the present study, we show that Mp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Mp1p was capable of detecting this protein from the cell culture supernatant of P. marneffei at 104 cells/ml. The anti-Mp1p antibody is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also specific for P. marneffei since the cell culture supernatants of the other three fungi gave negative results. Finally, a clinical evaluation of sera from penicilliosis patients indicates that 17 of 26 (65%) patients are Mp1p antigen test positive. Furthermore, a Mp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for P. marneffei carry a sensitive of 88% (23 of 26), with a positive predictive value of 100% and a negative predictive value of 96%. The specificities of the tests are high since none of the 85 control sera was positive by either test.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, Pathology Building, Queen Mary Hospital Compound, Hong Kong. Phone: (852) 2855-4822. Fax: (852) 2855-1241. E-mail: lcao{at}hkucc.Hku.hk.

Journal of Clinical Microbiology, April 1999, p. 981-986, Vol. 37, No. 4
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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