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Journal of Clinical Microbiology, May 1999, p. 1306-1312, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Phenotypic and Genomic Variation among Staphylococcus epidermidis Strains Infecting Joint Prostheses

Jacques-Olivier Galdbart,1,2 Anne Morvan,1 Nicole Desplaces,3 and Nevine el Solh1,*

Unité des Staphylocoques, National Reference Center for Staphylococci, Institut Pasteur, 72724 Paris Cedex 15,1 Service de Microbiologie, Hôpital Beaujon, A.P.-H.P., 92100 Clichy,2 and Service de Biologie, Hôpital de la Croix-Saint-Simon, 75020 Paris,3 France

Received 8 September 1998/Returned for modification 21 November 1998/Accepted 21 January 1999

We studied the SmaI and SstII macrorestriction patterns of 54 Staphylococcus epidermidis strains isolated from 14 patients infected following the implantation of joint prostheses. Multiple strains from pus and infected tissue specimens of each patient were selected on the basis of different colony morphologies and drug resistance patterns. The same criteria were used to select 23 S. epidermidis strains from hand swabs of eight healthy individuals. For 10 of the 14 patients, all the intrapatient strains appeared to be closely or possibly related, whereas related strains were detected in the skin flora of only one of the eight healthy individuals. This observation suggests that, in most cases, the patients were infected by a single S. epidermidis clone which subsequently underwent rearrangements that yielded derivatives with divergent phenotypes and, occasionally, divergent macrorestriction patterns. The four patients whose specimens contained unrelated S. epidermidis strains were probably infected with several polyclonal strains.

* Corresponding author. Mailing address: Unité des Staphylocoques, National Reference Center for Staphylococci, Institut Pasteur, 72724 Paris Cedex 15, France. Phone: (33) 01 45 68 83 63. Fax: (33) 01 40 61 31 63. E-mail: nelsolh{at}pasteur.fr.

Journal of Clinical Microbiology, May 1999, p. 1306-1312, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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