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Journal of Clinical Microbiology, May 1999, p. 1340-1347, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

GB Virus C/Hepatitis G Virus Groups and Subgroups: Classification by a Restriction Fragment Length Polymorphism Method Based on Phylogenetic Analysis of the 5' Untranslated Region

J. F. Quarleri,1,2 V. L. Mathet,1,2 M. Feld,1 D. Ferrario,1 M. P. della Latta,1 R. Verdun,3 D. O. Sánchez,3 and J. R. Oubiña1,2,*

Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires,1 Facultad de Medicina, Universidad del Salvador,2 and Instituto de Investigaciones Biotecnológicas, Universidad de San Martín,3 Buenos Aires, Argentina

Received 28 August 1998/Returned for modification 19 November 1998/Accepted 27 January 1999

A phylogenetic tree based on 150 5' untranslated region sequences deposited in GenBank database allowed segregation of the sequences into three major groups, including two subgroups, i.e., 1, 2a, 2b, and 3, supported by bootstrap analysis. Restriction site analysis of these sequences predicted that HinfI and either AatII or AciI could be used for genomic typing with 99.4% accuracy. cDNA sequencing and subsequent alignment of 21 Argentine GB virus C/hepatitis G virus strains confirmed restriction fragment length polymorphism patterns theoretically predicted. This method may be useful for a rapid screening of samples when either epidemiological or transmission studies of this agent are carried out.

* Corresponding author. Mailing address: Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 11, (1121) Buenos Aires, Argentina. Phone: 54-11-4508-3689. Fax: 54-11-4508-3705. E-mail: j_oubina{at}hotmail.com.

Journal of Clinical Microbiology, May 1999, p. 1340-1347, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.