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Journal of Clinical Microbiology, May 1999, p. 1503-1509, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Direct Quantification of the Enteric Bacterium Oxalobacter formigenes in Human Fecal Samples by Quantitative Competitive-Template PCR

H. Sidhu,1,* R. P. Holmes,2 M. J. Allison,3 and A. B. Peck4

Ixion Biotechnology, Inc., Alachua,1 and Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville,4 Florida; Department of Urology, Bowman Gray School of Medicine, Winston-Salem, North Carolina2; and National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa3

Received 2 October 1998/Returned for modification 8 December 1998/Accepted 26 January 1999

Homeostasis of oxalic acid appears to be regulated, in part, by the gut-associated bacterium Oxalobacter formigenes. The loss of this bacterium from the gut flora is associated with an increased susceptibility to hyperoxaluria, a condition which can lead to the formation of calcium oxalate crystalluria and kidney stones. In order to identify and quantify the presence of O. formigenes in clinical specimens, a quantitative-PCR-based assay system utilizing a competitive DNA template as an internal standard was developed. This quantitative competitive-template PCR test allows for the rapid, highly specific, and reproducible quantification of O. formigenes in fecal samples and provides a prototype for development of DNA-based quantitative assays for enteric bacteria.

* Corresponding author. Mailing address: Ixion Biotechnology, Inc., Biotechnology Development Institute, 13709 Progress Blvd., Box 13, Alachua, FL 32615. Phone: (904) 418-1429. Fax: (904) 418-1583. E-mail: sidhu{at}biotech.ufl.org.

Journal of Clinical Microbiology, May 1999, p. 1503-1509, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.