This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hussain, A. I. Articles by Gangemi, J. D. Search for Related Content PubMed PubMed Citation Articles by Hussain, A. I. Articles by Gangemi, J. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1999, p. 1518-1523, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mycoplasma penetrans and Other Mycoplasmas in Urine of Human Immunodeficiency Virus-Positive Children

Althaf I. Hussain,¹ William Lane M. Robson,² Robin Kelley,³ Tanya Reid,⁴ and J. David Gangemi^5,*

Department of Microbiology and Molecular Medicine, Clemson University, Clemson, South Carolina¹; Department of Pediatric Nephrology² and Department of Pediatric Infectious Disease,³ The Children's Hospital, Greenville Hospital System, and Greenville Hospital System/Clemson University Biomedical Cooperative,⁵ Greenville, South Carolina; and Children's Immunology Center,⁴ University of South Carolina School of Medicine,⁴ Columbia, South Carolina

Received 18 May 1998/Returned for modification 18 August 1998/Accepted 28 January 1999

Urine samples from children with human immunodeficiency virus (HIV) infection and healthy controls were examined for mycoplasmas by culture. Standard biochemical assays, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and PCR (16S and 16S-23S spacer rRNA region) were used for identification of isolates. Mycoplasmas were identified from 13 (87%) of 15 HIV-positive patients and 3 (20%) of 15 HIV-negative control patients. The frequency and type of mycoplasma varied with the severity of HIV infection. Mycoplasma penetrans, Mycoplasma pirum, Mycoplasma fermentans, and Mycoplasma genitalium were isolated from patients with severe immunodeficiency. Mycoplasma hominis and Ureaplasma urealyticum were isolated more frequently from children in the early stages of HIV infection and from HIV-negative patients. Mycoplasma penetrans was isolated from one (50%) of two patients in Centers for Disease Control and Prevention (CDC) group B and from five (55.5%) of nine pediatric patients with AIDS (CDC group C). This is the first report that indicates that "AIDS-associated" mycoplasmas are more common in HIV-infected children than in HIV-negative controls.

---

^* Corresponding author. Mailing address: Hollings Cancer Center, Prevention and Control, 261 Calhoun St., Suite 302, P.O. Box 250187, Clemson, SC 29425. Phone: (843) 876-1561. Fax: (843) 876-1963. E-mail: gangemij{at}musc.edu.

---

Journal of Clinical Microbiology, May 1999, p. 1518-1523, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Esaki Muthu Shankar, , Vignesh, R., Balakrishnan, P., Velu, V., Ponmalar, E., Murugavel, K. G., Saravanan, S., Nandagopal, P., Hayath, K., Solomon, S., Vengatesan, A., Usha Anand Rao, (2009). Cold Agglutinins in HIV-Seropositive Participants and Diagnosis of Respiratory Disease Due to Mycoplasma pneumoniae. J Int Assoc Physicians AIDS Care (Chic Ill) 8: 229-234 [Abstract]  
• Waites, K. B., Katz, B., Schelonka, R. L. (2005). Mycoplasmas and Ureaplasmas as Neonatal Pathogens. Clin. Microbiol. Rev. 18: 757-789 [Abstract] [Full Text]  
• Sasaki, Y., Ishikawa, J., Yamashita, A., Oshima, K., Kenri, T., Furuya, K., Yoshino, C., Horino, A., Shiba, T., Sasaki, T., Hattori, M. (2002). The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans. Nucleic Acids Res 30: 5293-5300 [Abstract] [Full Text]