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Journal of Clinical Microbiology, May 1999, p. 1561-1563, Vol. 37, No. 5
Servicio de Microbiología y
Parasitología,
Received 4 September 1998/Returned for modification 5 November
1998/Accepted 5 January 1999
Some techniques available at our laboratory were tested for their
ability to aid in the morphological diagnosis of hydatid elements
(Echinococcus granulosus ["Taenia
echinococcus"]) isolated from cysts in humans and sheep.
Unstained, methanol-fixed hooklets were fluorescent, most starkly so
under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-rhodamine and Gram
procedures failed to stain hooklets. Ziehl-Neelsen stain
yielded indifferent results when organisms were viewed under
transmitted light but resulted in a surprisingly intense red
fluorescence when organisms were viewed under green light (excitation,
546 nm; long pass, 590 nm). Wheatley trichrome stain gave better and
more uniform results than fuchsin. Ryan trichrome blue stain
was the best under transmitted light; hooklets stained uniformly and
intensely and were easily distinguishable from the background. Very
satisfactory results were also obtained with a much simpler procedure
(modified Baxby technique: no fixation, steaming hot 1% safranin for 2 min, and malachite green for 30 s). Therefore, Ryan and modified
Baxby stains are recommended for the examination of E. granulosus under transmitted light. For fluorescence microscopy,
Ziehl-Neelsen stain under green excitation light, or violet light with
no staining, is also very useful. Epifluorescence microscopy is
especially convenient for examining samples concentrated by
filtration, as it renders the filter pores inconspicuous.
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Visualization of Hydatid Elements: Comparison of Several
Techniques
*
Corresponding author. Mailing address: Servicio de
Microbiología y Parasitología, Hospital Clínico
Universitario, 50009 Zaragoza, Spain. Phone: 34-976 556 400, ext. 4319. Fax: 34-976 761 664. E-mail: aclavel{at}posta.unizar.es.
Journal of Clinical Microbiology, May 1999, p. 1561-1563, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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