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Journal of Clinical Microbiology, May 1999, p. 1579-1581, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of the Digene Hybrid Capture II CT-ID Test for Detection of Chlamydia trachomatis in Endocervical Specimens

Jennifer L. Girdner,1 Allison P. Cullen,2 Teresa G. Salama,3 Ling He,2 Attila Lorincz,2 and Thomas C. Quinn1,4,*

Division of Infectious Diseases, The Johns Hopkins University,1 and Baltimore City Health Department,3 Baltimore, Digene Corporation, Silver Spring,2 and National Institute of Allergy and Infectious Diseases, Bethesda,4 Maryland

Received 16 October 1998/Returned for modification 15 December 1998/Accepted 28 January 1999

The performance characteristics of the new signal amplification-based Hybrid Capture (HC) II CT-ID test system (Digene, Silver Spring, Md.) with endocervical specimens were compared to those of tissue culture and PCR (AMPLICOR CT PCR; Roche Molecular Systems, Branchburg, N.J.) for detection of Chlamydia trachomatis in 587 women. HC II CT-ID identified 62 of 65 confirmed C. trachomatis-positive patients (sensitivity of 95.4%) and was negative for 517 of 522 patients who were negative by culture and PCR (specificity of 99.0%). Twelve of the 65 confirmed positive patients were negative by culture but were identified by both HC II CT-ID and PCR (sensitivity of culture was 81.5% [P < 0.01]). In comparison, PCR detected 59 of 65 positive specimens (sensitivity of 90.8%) and had a specificity of 99.6% (520 of 522). These results demonstrate that the Digene HC II CT-ID test is a highly sensitive and specific assay for the detection of C. trachomatis infection in endocervical specimens.

* Corresponding author. Mailing address: The Johns Hopkins University, Division of Infectious Diseases, 720 Rutland Ave., Ross Research Building 1159, Baltimore, MD 21205. Phone: (410) 955-7635. Fax: (410) 614-9775. E-mail: tquinn{at}welchlink.welch.jhu.edu.

Journal of Clinical Microbiology, May 1999, p. 1579-1581, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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  • Solomon, A. W., Peeling, R. W., Foster, A., Mabey, D. C. W. (2004). Diagnosis and Assessment of Trachoma. Clin. Microbiol. Rev. 17: 982-1011 [Abstract] [Full Text]  
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