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Journal of Clinical Microbiology, May 1999, p. 1587-1590, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid PCR Test for Discriminating between Candida albicans and Candida dubliniensis Isolates Using Primers Derived from the pH-Regulated PHR1 and PHR2 Genes of C. albicans

Oliver Kurzai,1 Werner J. Heinz,1 Derek J. Sullivan,2 David C. Coleman,2 Matthias Frosch,1 and Fritz A. Mühlschlegel1,*

Institut für Hygiene und Mikrobiologie, Universität Würzburg, 97080 Würzburg, Germany,1 and Department of Oral Medicine and Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland2

Received 1 September 1998/Returned for modification 5 October 1998/Accepted 25 January 1999

The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regulated C. albicans PHR1 and PHR2 genes in C. dubliniensis. Restriction fragment length polymorphism analysis suggests that there are significant sequence differences between the genes of the two species. In order to exploit this apparent difference, oligonucleotide primers based on the coding sequence of the C. albicans PHR1 structural gene were designed and used in PCR experiments. Use of these primers with C. albicans template DNA from 17 strains yielded a predicted 1.6-kb product, while C. dubliniensis template DNA from 19 strains yielded no product. We therefore propose that PCR using these primers is a rapid and reliable means of distinguishing the two germ tube- and chlamydospore-producing species C. albicans and C. dubliniensis.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie, University Würzburg, Josef-Schneider-Straße 2, 97080 Würzburg, Germany. Phone: (0931) 201-3901. Fax: (0931) 201-3445. E-mail: fmuehlschlegel{at}hygiene.uni-wuerzburg.de.

Journal of Clinical Microbiology, May 1999, p. 1587-1590, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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