This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cavassini, M.
Right arrow Articles by Bille, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cavassini, M.
Right arrow Articles by Bille, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1999, p. 1591-1594, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of MRSA-Screen, a Simple Anti-PBP 2a Slide Latex Agglutination Kit, for Rapid Detection of Methicillin Resistance in Staphylococcus aureus

Matthias Cavassini,1 Aline Wenger,1 Katia Jaton,1 Dominique S. Blanc,2 and Jacques Bille1,*

Institut de Microbiologie1 and Division Autonome de Médecine Préventive Hospitalière,2 Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland

Received 8 September 1998/Returned for modification 2 November 1998/Accepted 7 February 1999

The MRSA-Screen test (Denka Seiken Co., Ltd., Tokyo, Japan), consisting of a slide latex agglutination kit that detects PBP 2a with a monoclonal antibody, was blindly compared to the oxacillin disk diffusion test, the oxacillin-salt agar screen, and PCR of the mecA gene for the detection of methicillin resistance in Staphylococcus aureus. A total of 120 methicillin-susceptible S. aureus (MSSA) and 80 methicillin-resistant S. aureus (MRSA) isolates, defined by the absence or presence of the mecA gene, respectively, were tested. The MRSA-Screen test, the oxacillin disk diffusion test, and the oxacillin-salt agar screening test showed sensitivities of 100, 61.3, and 82.5% and specificities of 99.2, 96.7, and 98.3%, respectively. We conclude that the MRSA-Screen is a very accurate, reliable, and fast test (15 min) for differentiation of MRSA from MSSA colonies on agar plates.

* Corresponding author. Mailing address: Institut de Microbiologie, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland. Phone: 0041 21 314 40 57. Fax: 0041 21 314 40 60. E-mail: jacques.bille{at}chuv.hospvd.ch.

Journal of Clinical Microbiology, May 1999, p. 1591-1594, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Stenholm, T., Hakanen, A. J., Vaarno, J., Pihlasalo, S., Terho, P., Hanninen, P. E., Vuopio-Varkila, J., Huovinen, P., Kotilainen, P. (2009). Methicillin-Resistant Staphylococcus aureus Screening by Online Immunometric Monitoring of Bacterial Growth under Selective Pressure. Antimicrob. Agents Chemother. 53: 5088-5094 [Abstract] [Full Text]  
  • Mencacci, A., Montecarlo, I., Gonfia, F., Moretti, A., Cardaccia, A., Farinelli, S., Pagliochini, M. R., Giuliani, A., Basileo, M., Pasticci, M. B., Bistoni, F. (2009). Comparison of the BD Phoenix System with the Cefoxitin Disk Diffusion Test for Detection of Methicillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci. J. Clin. Microbiol. 47: 2288-2291 [Abstract] [Full Text]  
  • Sauer, P., Sila, J., Stosova, T., Vecerova, R., Hejnar, P., Vagnerova, I., Kolar, M., Raclavsky, V., Petrzelova, J., Loveckova, Y., Koukalova, D. (2008). Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic. J Med Microbiol 57: 403-410 [Abstract] [Full Text]  
  • Weist, K., Cimbal, A.-K., Lecke, C., Kampf, G., Ruden, H., Vonberg, R.-P. (2006). Evaluation of six agglutination tests for Staphylococcus aureus identification depending upon local prevalence of meticillin-resistant S. aureus (MRSA).. J Med Microbiol 55: 283-290 [Abstract] [Full Text]  
  • Brown, D. F. J., Edwards, D. I., Hawkey, P. M., Morrison, D., Ridgway, G. L., Towner, K. J., Wren, M. W. D., on behalf of the Joint Working Party of the Britis, (2005). Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56: 1000-1018 [Abstract] [Full Text]  
  • Bressler, A. M., Williams, T., Culler, E. E., Zhu, W., Lonsway, D., Patel, J. B., Nolte, F. S. (2005). Correlation of Penicillin Binding Protein 2a Detection with Oxacillin Resistance in Staphylococcus aureus and Discovery of a Novel Penicillin Binding Protein 2a Mutation. J. Clin. Microbiol. 43: 4541-4544 [Abstract] [Full Text]  
  • Swenson, J. M., Tenover, F. C., the Cefoxitin Disk Study Group, (2005). Results of Disk Diffusion Testing with Cefoxitin Correlate with Presence of mecA in Staphylococcus spp.. J. Clin. Microbiol. 43: 3818-3823 [Abstract] [Full Text]  
  • Kaplan, S., Marlowe, E. M., Hogan, J. J., Doymaz, M., Bruckner, D. A., Simor, A. E. (2005). Sensitivity and Specificity of a Rapid rRNA Gene Probe Assay for Simultaneous Identification of Staphylococcus aureus and Detection of mecA. J. Clin. Microbiol. 43: 3438-3442 [Abstract] [Full Text]  
  • Miller, M. B., Meyer, H., Rogers, E., Gilligan, P. H. (2005). Comparison of Conventional Susceptibility Testing, Penicillin-Binding Protein 2a Latex Agglutination Testing, and mecA Real-Time PCR for Detection of Oxacillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococcus. J. Clin. Microbiol. 43: 3450-3452 [Abstract] [Full Text]  
  • Kipp, F., Becker, K., Peters, G., von Eiff, C. (2004). Evaluation of Different Methods To Detect Methicillin Resistance in Small-Colony Variants of Staphylococcus aureus. J. Clin. Microbiol. 42: 1277-1279 [Abstract] [Full Text]  
  • Chapin, K. C., Musgnug, M. C. (2004). Evaluation of Penicillin Binding Protein 2a Latex Agglutination Assay for Identification of Methicillin-Resistant Staphylococcus aureus Directly from Blood Cultures. J. Clin. Microbiol. 42: 1283-1284 [Abstract] [Full Text]  
  • Ferreira, R. B. R., Iorio, N. L. P., Malvar, K. L., Nunes, A. P. F., Fonseca, L. S., Bastos, C. C. R., Santos, K. R. N. (2003). Coagulase-Negative Staphylococci: Comparison of Phenotypic and Genotypic Oxacillin Susceptibility Tests and Evaluation of the Agar Screening Test by Using Different Concentrations of Oxacillin. J. Clin. Microbiol. 41: 3609-3614 [Abstract] [Full Text]  
  • Francois, P., Pittet, D., Bento, M., Pepey, B., Vaudaux, P., Lew, D., Schrenzel, J. (2003). Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay. J. Clin. Microbiol. 41: 254-260 [Abstract] [Full Text]  
  • Felten, A., Grandry, B., Lagrange, P. H., Casin, I. (2002). Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA): a Disk Diffusion Method with Cefoxitin and Moxalactam, the Vitek 2 System, and the MRSA-Screen Latex Agglutination Test. J. Clin. Microbiol. 40: 2766-2771 [Abstract] [Full Text]  
  • Jonas, D., Speck, M., Daschner, F. D., Grundmann, H. (2002). Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs. J. Clin. Microbiol. 40: 1821-1823 [Abstract] [Full Text]  
  • Sakoulas, G., Gold, H. S., Venkataraman, L., DeGirolami, P. C., Eliopoulos, G. M., Qian, Q. (2001). Methicillin-Resistant Staphylococcus aureus: Comparison of Susceptibility Testing Methods and Analysis of mecA-Positive Susceptible Strains. J. Clin. Microbiol. 39: 3946-3951 [Abstract] [Full Text]  
  • Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Mack, D. (2001). Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci by a Penicillin-Binding Protein 2a-Specific Latex Agglutination Test. J. Clin. Microbiol. 39: 3700-3702 [Abstract] [Full Text]  
  • Swenson, J. M., Spargo, J., Tenover, F. C., Ferraro, M. J. (2001). Optimal Inoculation Methods and Quality Control for the NCCLS Oxacillin Agar Screen Test for Detection of Oxacillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 39: 3781-3784 [Abstract] [Full Text]  
  • Swenson, J. M., Williams, P. P., Killgore, G., O'Hara, C. M., Tenover, F. C. (2001). Performance of Eight Methods, Including Two New Rapid Methods, for Detection of Oxacillin Resistance in a Challenge Set of Staphylococcus aureus Organisms. J. Clin. Microbiol. 39: 3785-3788 [Abstract] [Full Text]  
  • Yamazumi, T., Marshall, S. A., Wilke, W. W., Diekema, D. J., Pfaller, M. A., Jones, R. N. (2001). Comparison of the Vitek Gram-Positive Susceptibility 106 Card and the MRSA-Screen Latex Agglutination Test for Determining Oxacillin Resistance in Clinical Bloodstream Isolates of Staphylococcus aureus. J. Clin. Microbiol. 39: 53-56 [Abstract] [Full Text]  
  • Zbinden, R., Ritzler, M., Ritzler, E., Berger-Bächi, B. (2001). Detection of Penicillin-Binding Protein 2a by Rapid Slide Latex Agglutination Test in Coagulase-Negative Staphylococci. J. Clin. Microbiol. 39: 412-412 [Full Text]  
  • Hussain, Z., Stoakes, L., Garrow, S., Longo, S., Fitzgerald, V., Lannigan, R. (2000). Rapid Detection of mecA-Positive and mecA-Negative Coagulase-Negative Staphylococci by an Anti-Penicillin Binding Protein 2a Slide Latex Agglutination Test. J. Clin. Microbiol. 38: 2051-2054 [Abstract] [Full Text]  
  • Louie, L., Matsumura, S. O., Choi, E., Louie, M., Simor, A. E. (2000). Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus. J. Clin. Microbiol. 38: 2170-2173 [Abstract] [Full Text]  
  • Hussain, Z., Stoakes, L., Massey, V., Diagre, D., Fitzgerald, V., El Sayed, S., Lannigan, R. (2000). Correlation of Oxacillin MIC with mecA Gene Carriage in Coagulase-Negative Staphylococci. J. Clin. Microbiol. 38: 752-754 [Abstract] [Full Text]  
  • Marriott, D. J. E., Karagiannis, T., Harkness, J. L., Kearney;, P., Cavassini, M., Wenger, A., Jaton, K., Bille, J., Blanc, D. S. (1999). Further Evaluation of the MRSA-Screen Kit for Rapid Detection of Methicillin Resistance. J. Clin. Microbiol. 37: 3783-3784 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»