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Journal of Clinical Microbiology, May 1999, p. 1619-1620, Vol. 37, No. 5
Hospital de Base, Brasilia,
Brazil,1 and Miriam
Hospital2 and the Veterans
Administration Medical Center,3 Providence,
Rhode Island
Received 14 October 1998/Returned for modification 14 January
1999/Accepted 9 February 1999
Eight Staphylococcus aureus strains initially
identified by Vitek GPS-BS or GPS-SA cards as resistant to oxacillin,
but susceptible to most non-beta-lactam antibiotics, were found on
further testing to be susceptible to oxacillin and ceftizoxime by disk
diffusion tests. For all these strains, the MICs of oxacillin were
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Misclassification of Susceptible Strains of
Staphylococcus aureus as Methicillin-Resistant S. aureus by a Rapid Automated Susceptibility Testing
System
0.5 µg/ml by agar dilution tests, and the strains were
oxacillin susceptible when tested by the BBL Crystal MRSA ID and a
Vitek machine with GPS-101 cards. None grew on oxacillin-salt agar
screening plates. None were positive for mecA gene
sequences by PCR. When S. aureus strains tested by Vitek
GPS-SA or GPS-BS cards appear resistant to only penicillin and
oxacillin, a confirmatory test such as the oxacillin-salt agar
screening method should be performed.
*
Corresponding author. Mailing address: Miriam Hospital,
Providence, R.I. Phone: (401) 793-4620. Fax: (401) 751-2398. E-mail: john_boyce{at}brown.edu.
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