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Journal of Clinical Microbiology, May 1999, p. 1619-1620, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Misclassification of Susceptible Strains of Staphylococcus aureus as Methicillin-Resistant S. aureus by a Rapid Automated Susceptibility Testing System

Julival Ribeiro,1 F. D. Vieira,1 Tom King,2 Julia B. D'Arezzo,3 and John M. Boyce2,*

Hospital de Base, Brasilia, Brazil,1 and Miriam Hospital2 and the Veterans Administration Medical Center,3 Providence, Rhode Island

Received 14 October 1998/Returned for modification 14 January 1999/Accepted 9 February 1999

Eight Staphylococcus aureus strains initially identified by Vitek GPS-BS or GPS-SA cards as resistant to oxacillin, but susceptible to most non-beta-lactam antibiotics, were found on further testing to be susceptible to oxacillin and ceftizoxime by disk diffusion tests. For all these strains, the MICs of oxacillin were <= 0.5 µg/ml by agar dilution tests, and the strains were oxacillin susceptible when tested by the BBL Crystal MRSA ID and a Vitek machine with GPS-101 cards. None grew on oxacillin-salt agar screening plates. None were positive for mecA gene sequences by PCR. When S. aureus strains tested by Vitek GPS-SA or GPS-BS cards appear resistant to only penicillin and oxacillin, a confirmatory test such as the oxacillin-salt agar screening method should be performed.

* Corresponding author. Mailing address: Miriam Hospital, Providence, R.I. Phone: (401) 793-4620. Fax: (401) 751-2398. E-mail: john_boyce{at}brown.edu.

Journal of Clinical Microbiology, May 1999, p. 1619-1620, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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