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Journal of Clinical Microbiology, May 1999, p. 1621-1624, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Simultaneous Detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA Gene-Directed Multiplex PCR

Georg Conrads,1,2,* Thomas F. Flemmig,3 Ilse Seyfarth,1 Friedrich Lampert,1 and Rudolf Lütticken2

Clinic of Conservative and Preventive Dentistry and Periodontology,1 and Department of Medical Microbiology,2 University Hospital (RWTH), D-52057 Aachen, and Department of Periodontology, Julius Maximilian University, D-97070 Würzburg,3 Germany

Received 31 July 1998/Returned for modification 2 November 1998/Accepted 13 February 1999

In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.

* Corresponding author. Mailing address: Institute of Medical Microbiology, University Hospital (RWTH), Pauwelsstrasse 30, D-52057 Aachen, Germany. Phone: (49)-241-8089787. Fax: (49)-241-8888483. E-mail: conrads{at}alpha.imib.rwth-aachen.de.

Journal of Clinical Microbiology, May 1999, p. 1621-1624, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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