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Journal of Clinical Microbiology, May 1999, p. 1625-1627, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Disk Diffusion Method for Determining Susceptibilities of Candida spp. to MK-0991

Mario Lozano-Chiu,* Page W. Nelson, Victor L. Paetznick, and John H. Rex

Division of Infectious Diseases, Department of Internal Medicine, Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas 77030

Received 27 October 1998/Returned for modification 17 December 1998/Accepted 2 February 1999

We have developed an agar-based methodology for testing susceptibilities of Candida spp. to the new antifungal agent MK-0991, a glucan synthase inhibitor. Results obtained with this method correlated well with the results obtained by the National Committee for Clinical Laboratory Standards M27-A broth microdilution reference method. However, as noted with prior comparisons of broth- and agar-based systems, some isolates yielded inhibition zones which were not consistent with the MICs obtained for them. Understanding the implications of these differences will require testing in an in vivo system.

* Corresponding author. Present address: Departamento de Investigación, Glaxo Wellcome, S.A., Parque Tecnológico de Madrid, C/Severo Ochoa 2, 28760 Tres Cantos, Madrid, Spain. Phone: 34 91 8070301. Fax: 34 91 8070595. E-mail: mlc34906{at}GlaxoWellcome.co.uk.

Journal of Clinical Microbiology, May 1999, p. 1625-1627, Vol. 37, No. 5
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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