This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Mahony, J.
Right arrow Articles by Hill, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Mahony, J.
Right arrow Articles by Hill, C.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1999, p. 1699-1703, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

VP4 and VP7 Genotyping of Rotavirus Samples Recovered from Infected Children in Ireland over a 3-Year Period

Jim O'Mahony,1,2 Barbara Foley,1,2 Sheila Morgan,1,2 John G. Morgan,1 and Colin Hill1,2,*

Department of Microbiology1 and National Food Biotechnology Centre,2 University College, Cork, Ireland

Received 21 December 1998/Returned for modification 4 February 1999/Accepted 23 February 1999

Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive specimens collected from nine hospitals we successfully identified the P type in 182 (94%) of the samples and the G type in 165 (85.5%) of the samples. Only four samples could not be assigned a G or P type. Two P types existed in Ireland, P[8] (78%) and P[4] (16%), and their relative incidence varied over the 3 years of this study. No P[6] or P[9] types were detected. G1 was the most predominant G type (55%), and the incidences of G2, G3, and G4 isolates were 15.5, 1, and 11%, respectively. Three percent of the samples tested had a mixed G type. A P and G type was assigned to 158 (81.8%) of samples. Of the typeable samples, G1 P[8] was the most prevalent (65%), whereas G2 P[4] (17%), G3 P[8] (1%), G4 P[8] (12%), and mixed types (all G1/ G4 P[8]) (4%) were detected less frequently. In the third year a significant genotypic shift from G1 P[8] to G2 P[4] and G4 P[8] was observed. During the study, we noticed that the inclusion of random primers during cDNA synthesis greatly increased the specificity of the PCR typing assays. No correlation was seen between the contributing hospitals and a specific genotype. In conclusion, the coverage of infection given by the recently licensed tetravalent vaccine would be significantly high in Ireland, although future monitoring of genotypic changes among Irish isolates should be encouraged.

* Corresponding author. Mailing address: Microbiology Department, University College Cork, College Rd., Cork, Ireland. Phone: 353-21-902397. Fax: 353-21-903101. E-mail: c.hill{at}ucc.ie.

Journal of Clinical Microbiology, June 1999, p. 1699-1703, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kiulia, N. M., Nyaundi, J. K., Peenze, I., Nyachieo, A., Musoke, R. N., Steele, A. D., Mwenda, J. M. (2009). Rotavirus Infections among HIV-Infected Children in Nairobi, Kenya. J Trop Pediatr 55: 318-323 [Abstract] [Full Text]  
  • Mascarenhas, J. D. P., Leite, J. P. G., Lima, J. C., Heinemann, M. B., Oliveira, D. S., Araujo, I. T., Soares, L. S., Gusmao, R. H. P., Gabbay, Y. B., Linhares, A. C. (2007). Detection of a neonatal human rotavirus strain with VP4 and NSP4 genes of porcine origin. J Med Microbiol 56: 524-532 [Abstract] [Full Text]  
  • Bucardo, F., Karlsson, B., Nordgren, J., Paniagua, M., Gonzalez, A., Amador, J. J., Espinoza, F., Svensson, L. (2007). Mutated G4P[8] Rotavirus Associated with a Nationwide Outbreak of Gastroenteritis in Nicaragua in 2005. J. Clin. Microbiol. 45: 990-997 [Abstract] [Full Text]  
  • Logan, C., O'Leary, J. J., O'Sullivan, N. (2006). Real-Time Reverse Transcription-PCR for Detection of Rotavirus and Adenovirus as Causative Agents of Acute Viral Gastroenteritis in Children.. J. Clin. Microbiol. 44: 3189-3195 [Abstract] [Full Text]  
  • Min, B. S., Noh, Y. J., Shin, J. H., Baek, S. Y., Kim, J. O., Min, K. I., Ryu, S. R., Kim, B. G., Kim, D. K., Lee, S. H., Min, H. K., Ahn, B. Y., Park, S. N. (2004). Surveillance Study (2000 to 2001) of G- and P-Type Human Rotaviruses Circulating in South Korea. J. Clin. Microbiol. 42: 4297-4299 [Abstract] [Full Text]  
  • Sanchez-Fauquier, A., Wilhelmi, I., Colomina, J., Cubero, E., Roman, E. (2004). Diversity of Group A Human Rotavirus Types Circulating over a 4-Year Period in Madrid, Spain. J. Clin. Microbiol. 42: 1609-1613 [Abstract] [Full Text]  
  • Santos, N., Soares, C. C., Volotao, E. M., Albuquerque, M. C. M., Hoshino, Y. (2003). Surveillance of Rotavirus Strains in Rio de Janeiro, Brazil, from 1997 to 1999. J. Clin. Microbiol. 41: 3399-3402 [Abstract] [Full Text]  
  • O'Halloran, F., Lynch, M., Cryan, B., Fanning, S. (2002). Application of Restriction Fragment Length Polymorphism Analysis of VP7-Encoding Genes: Fine Comparison of Irish and Global Rotavirus Isolates. J. Clin. Microbiol. 40: 524-531 [Abstract] [Full Text]  
  • Das, S., Sen, A., Uma, G., Varghese, V., Chaudhuri, S., Bhattacharya, S. K., Krishnan, T., Dutta, P., Dutta, D., Bhattacharya, M. K., Mitra, U., Kobayashi, N., Naik, T. N. (2002). Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern India. J. Clin. Microbiol. 40: 146-149 [Abstract] [Full Text]  
  • Bishop, R. F., Masendycz, P. J., Bugg, H. C., Carlin, J. B., Barnes, G. L. (2001). Epidemiological Patterns of Rotaviruses Causing Severe Gastroenteritis in Young Children throughout Australia from 1993 to 1996. J. Clin. Microbiol. 39: 1085-1091 [Abstract] [Full Text]  
  • O'Halloran, F., Lynch, M., Cryan, B., O'Shea, H., Fanning, S. (2000). Molecular Characterization of Rotavirus in Ireland: Detection of Novel Strains Circulating in the Population. J. Clin. Microbiol. 38: 3370-3374 [Abstract] [Full Text]  
  • Bon, F., Fromantin, C., Aho, S., Pothier, P., Kohli, E., The Azay Group, (2000). G and P Genotyping of Rotavirus Strains Circulating in France over a Three-Year Period: Detection of G9 and P[6] Strains at Low Frequencies. J. Clin. Microbiol. 38: 1681-1683 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»