This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pai, H.
Right arrow Articles by Choe, K. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pai, H.
Right arrow Articles by Choe, K. W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1999, p. 1758-1763, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Survey of Extended-Spectrum beta -Lactamases in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae: Prevalence of TEM-52 in Korea

Hyunjoo Pai,1,* Sen Lyu,1 Ji Hyang Lee,1 Jungmin Kim,2 Youngmi Kwon,2 Jong-Won Kim,3 and Kang Won Choe4

Division of Infectious Disease, Department of Internal Medicine,1 and Department of Microbiology,2 College of Medicine, University of Dankook, Chonan, and Department of Clinical Pathology, Sungkyunkwan University College of Medicine,3 and Department of Internal Medicine, Seoul National University College of Medicine,4 Seoul, Korea

Received 4 November 1998/Returned for modification 16 January 1999/Accepted 24 February 1999

Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum beta -lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain produced SHV-12, 1 strain produced a CMY-1-like enzyme, and 1 strain expressed SHV-2a and a CMY-1-like enzyme. Another two strains for which the MICs of ceftazidime and cefoxitin were high, were probable AmpC enzyme hyperproducers. Because of the high prevalence of TEM-52 in E. coli isolates, we further investigated the TEM-type ESBLs produced by Klebsiella pneumoniae in order to observe the distribution of TEM-52 enzymes among Enterobacteriaceae in Korea. All TEM enzymes produced by 12 strains of K. pneumoniae were identified as TEM-52. To evaluate the genetic relatedness among the organisms, ribotyping of TEM-52-producing E. coli and K. pneumoniae was performed. The ribotyping profiles of the organisms showed similar but clearly different patterns. In conclusion, TEM-52 is the most prevalent TEM-type ESBL in Korea.

* Corresponding author. Mailing address: Division of Infectious Disease, Department of Internal Medicine, College of Medicine, University of Dankook, San 29, Anseo-dong, Chonan, Chungnam, 330-715, Korea. Phone: 82-417-550-3918. Fax: 82-417-556-3256. E-mail: paihj{at}unitel.co.kr.

Journal of Clinical Microbiology, June 1999, p. 1758-1763, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Song, W., Lee, H., Lee, K., Jeong, S. H., Bae, I. K., Kim, J.-S., Kwak, H.-S. (2009). CTX-M-14 and CTX-M-15 enzymes are the dominant type of extended-spectrum {beta}-lactamase in clinical isolates of Escherichia coli from Korea. J Med Microbiol 58: 261-266 [Abstract] [Full Text]  
  • Kiratisin, P., Apisarnthanarak, A., Laesripa, C., Saifon, P. (2008). Molecular Characterization and Epidemiology of Extended-Spectrum- {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates Causing Health Care-Associated Infection in Thailand, Where the CTX-M Family Is Endemic. Antimicrob. Agents Chemother. 52: 2818-2824 [Abstract] [Full Text]  
  • Pai, H., Seo, M.-R., Choi, T. Y. (2007). Association of QnrB Determinants and Production of Extended-Spectrum {beta}-Lactamases or Plasmid-Mediated AmpC {beta}-Lactamases in Clinical Isolates of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 51: 366-368 [Abstract] [Full Text]  
  • Ryoo, N. H., Kim, E.-C., Hong, S. G., Park, Y. J., Lee, K., Bae, I. K., Song, E. H., Jeong, S. H. (2005). Dissemination of SHV-12 and CTX-M-type extended-spectrum {beta}-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea. J Antimicrob Chemother 56: 698-702 [Abstract] [Full Text]  
  • Kim, J., Lim, Y.-M., Jeong, Y.-S., Seol, S.-Y. (2005). Occurrence of CTX-M-3, CTX-M-15, CTX-M-14, and CTX-M-9 Extended-Spectrum {beta}-Lactamases in Enterobacteriaceae Clinical Isolates in Korea. Antimicrob. Agents Chemother. 49: 1572-1575 [Abstract] [Full Text]  
  • Kim, S., Kim, J., Kang, Y., Park, Y., Lee, B. (2004). Occurrence of Extended-Spectrum {beta}-Lactamases in Members of the Genus Shigella in the Republic of Korea. J. Clin. Microbiol. 42: 5264-5269 [Abstract] [Full Text]  
  • Pai, H., Kang, C.-I., Byeon, J.-H., Lee, K.-D., Park, W. B., Kim, H.-B., Kim, E.-C., Oh, M.-d., Choe, K.-W. (2004). Epidemiology and Clinical Features of Bloodstream Infections Caused by AmpC-Type-{beta}-Lactamase-Producing Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48: 3720-3728 [Abstract] [Full Text]  
  • Pai, H., Hong, J. Y., Byeon, J.-H., Kim, Y.-K., Lee, H.-J. (2004). High Prevalence of Extended-Spectrum {beta}-Lactamase-Producing Strains among Blood Isolates of Enterobacter spp. Collected in a Tertiary Hospital during an 8-Year Period and Their Antimicrobial Susceptibility Patterns. Antimicrob. Agents Chemother. 48: 3159-3161 [Abstract] [Full Text]  
  • Jeong, S. H., Bae, I. K., Lee, J. H., Sohn, S. G., Kang, G. H., Jeon, G. J., Kim, Y. H., Jeong, B. C., Lee, S. H. (2004). Molecular Characterization of Extended-Spectrum Beta-Lactamases Produced by Clinical Isolates of Klebsiella pneumoniae and Escherichia coli from a Korean Nationwide Survey. J. Clin. Microbiol. 42: 2902-2906 [Abstract] [Full Text]  
  • Yan, J.-J., Ko, W.-C., Wu, J.-J., Tsai, S.-H., Chuang, C.-L. (2004). Epidemiological Investigation of Bloodstream Infections by Extended Spectrum Cephalosporin-Resistant Escherichia coli in a Taiwanese Teaching Hospital. J. Clin. Microbiol. 42: 3329-3332 [Abstract] [Full Text]  
  • Weill, F.-X., Demartin, M., Fabre, L., Grimont, P. A. D. (2004). Extended-Spectrum-{beta}-Lactamase (TEM-52)-Producing Strains of Salmonella enterica of Various Serotypes Isolated in France. J. Clin. Microbiol. 42: 3359-3362 [Abstract] [Full Text]  
  • Mulvey, M. R., Bryce, E., Boyd, D., Ofner-Agostini, M., Christianson, S., Simor, A. E., Paton, S. (2004). Ambler Class A Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Klebsiella spp. in Canadian Hospitals. Antimicrob. Agents Chemother. 48: 1204-1214 [Abstract] [Full Text]  
  • Yan, J.-J., Hong, C.-Y., Ko, W.-C., Chen, Y.-J., Tsai, S.-H., Chuang, C.-L., Wu, J.-J. (2004). Dissemination of blaCMY-2 among Escherichia coli Isolates from Food Animals, Retail Ground Meats, and Humans in Southern Taiwan. Antimicrob. Agents Chemother. 48: 1353-1356 [Abstract] [Full Text]  
  • Yu, W.-L., Cheng, K.-C., Wu, L.-T., Pfaller, M. A., Winokur, P. L., Jones, R. N. (2004). Emergence of Two Klebsiella pneumoniae Isolates Harboring Plasmid-Mediated CTX-M-15 {beta}-Lactamase in Taiwan. Antimicrob. Agents Chemother. 48: 362-363 [Full Text]  
  • Jeong, Y. S., Lee, J. C., Kang, H. Y., Yu, H. S., Lee, E. Y., Choi, C. H., Tae, S. H., Lee, Y. C., Cho, D. T., Seol, S. Y. (2003). Epidemiology of Nalidixic Acid Resistance and TEM-1- and TEM-52-Mediated Ampicillin Resistance of Shigella sonnei Isolates Obtained in Korea between 1980 and 2000. Antimicrob. Agents Chemother. 47: 3719-3723 [Abstract] [Full Text]  
  • Lee, K., Yong, D., Yum, J. H., Kim, H. H., Chong, Y. (2003). Diversity of TEM-52 extended-spectrum {beta}-lactamase-producing non-typhoidal Salmonella isolates in Korea. J Antimicrob Chemother 52: 493-496 [Abstract] [Full Text]  
  • Melano, R., Corso, A., Petroni, A., Centron, D., Orman, B., Pereyra, A., Moreno, N., Galas, M. (2003). Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum {beta}-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina. J Antimicrob Chemother 52: 36-42 [Abstract] [Full Text]  
  • Pai, H., Byeon, J.-h., Yu, S., Lee, B. K., Kim, S. (2003). Salmonella enterica Serovar Typhi Strains Isolated in Korea Containing a Multidrug Resistance Class 1 Integron. Antimicrob. Agents Chemother. 47: 2006-2008 [Abstract] [Full Text]  
  • Wang, H., Kelkar, S., Wu, W., Chen, M., Quinn, J. P. (2003). Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum {beta}-Lactamases: Prevalence of CTX-M-3 at a Hospital in China. Antimicrob. Agents Chemother. 47: 790-793 [Abstract] [Full Text]  
  • Perilli, M., Segatore, B., De Massis, M. R., Pagani, L., Luzzaro, F., Rossolini, G. M., Amicosante, G. (2002). Biochemical Characterization of TEM-92 Extended-Spectrum {beta}-Lactamase, a Protein Differing from TEM-52 in the Signal Peptide. Antimicrob. Agents Chemother. 46: 3981-3983 [Abstract] [Full Text]  
  • Yan, J.-J., Ko, W.-C., Jung, Y.-C., Chuang, C.-L., Wu, J.-J. (2002). Emergence of Klebsiella pneumoniae Isolates Producing Inducible DHA-1 {beta}-Lactamase in a University Hospital in Taiwan. J. Clin. Microbiol. 40: 3121-3126 [Abstract] [Full Text]  
  • Kim, Y.-K., Pai, H., Lee, H.-J., Park, S.-E., Choi, E.-H., Kim, J., Kim, J.-H., Kim, E.-C. (2002). Bloodstream Infections by Extended-Spectrum {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Children: Epidemiology and Clinical Outcome. Antimicrob. Agents Chemother. 46: 1481-1491 [Abstract] [Full Text]  
  • Chanawong, A., M'Zali, F. H., Heritage, J., Xiong, J.-H., Hawkey, P. M. (2002). Three Cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China. Antimicrob. Agents Chemother. 46: 630-637 [Abstract] [Full Text]  
  • Kim, J., Shin, H.-S., Seol, S.-Y., Cho, D.-T. (2002). Relationship between blaSHV-12 and blaSHV-2a in Korea. J Antimicrob Chemother 49: 261-267 [Abstract] [Full Text]  
  • Perilli, M., Dell'Amico, E., Segatore, B., de Massis, M. R., Bianchi, C., Luzzaro, F., Rossolini, G. M., Toniolo, A., Nicoletti, G., Amicosante, G. (2002). Molecular Characterization of Extended-Spectrum {beta}-Lactamases Produced by Nosocomial Isolates of Enterobacteriaceae from an Italian Nationwide Survey. J. Clin. Microbiol. 40: 611-614 [Abstract] [Full Text]  
  • Chanawong, A., M'Zali, F. H., Heritage, J., Lulitanond, A., Hawkey, P. M. (2001). SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum {beta}-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand. J Antimicrob Chemother 48: 839-852 [Abstract] [Full Text]  
  • Pai, H., Lee, H.-J., Choi, E.-H., Kim, J., Jacoby, G. A. (2001). Evolution of TEM-Related Extended-Spectrum beta -Lactamases in Korea. Antimicrob. Agents Chemother. 45: 3651-3653 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Lee, K., Lim, J. K., Yong, D., Yum, J., Chong, Y., Okamoto, R., Inoue, M. (2001). Evaluation of Efficiency of Screening Extended-Spectrum {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Hospitals Where the Bacteria Are Increasingly Prevalent. J. Clin. Microbiol. 39: 3696-3699 [Abstract] [Full Text]  
  • Pai, H., Choi, E.-H., Lee, H.-J., Hong, J. Y., Jacoby, G. A. (2001). Identification of CTX-M-14 Extended-Spectrum {beta}-Lactamase in Clinical Isolates of Shigella sonnei, Escherichia coli, and Klebsiella pneumoniae in Korea. J. Clin. Microbiol. 39: 3747-3749 [Abstract] [Full Text]  
  • de Champs, C., Monne, C., Bonnet, R., Sougakoff, W., Sirot, D., Chanal, C., Sirot, J. (2001). New TEM Variant (TEM-92) Produced by Proteus mirabilis and Providencia stuartii Isolates. Antimicrob. Agents Chemother. 45: 1278-1280 [Abstract] [Full Text]  
  • Girlich, D., Poirel, L., Leelaporn, A., Karim, A., Tribuddharat, C., Fennewald, M., Nordmann, P. (2001). Molecular Epidemiology of the Integron-Located VEB-1 Extended-Spectrum {beta}-Lactamase in Nosocomial Enterobacterial Isolates in Bangkok, Thailand. J. Clin. Microbiol. 39: 175-182 [Abstract] [Full Text]  
  • Teshager, T., Domínguez, L., Moreno, M. A., Saénz, Y., Torres, C., Cardeñosa, S. (2000). Isolation of an SHV-12 beta -Lactamase-Producing Escherichia coli Strain from a Dog with Recurrent Urinary Tract Infections. Antimicrob. Agents Chemother. 44: 3483-3484 [Full Text]  
  • Yan, J.-J., Ko, W.-C., Tsai, S.-H., Wu, H.-M., Jin, Y.-T., Wu, J.-J. (2000). Dissemination of CTX-M-3 and CMY-2 beta -Lactamases among Clinical Isolates of Escherichia coli in Southern Taiwan. J. Clin. Microbiol. 38: 4320-4325 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»