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Journal of Clinical Microbiology, June 1999, p. 1758-1763, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Survey of Extended-Spectrum
-Lactamases in
Clinical Isolates of Escherichia coli and Klebsiella
pneumoniae: Prevalence of TEM-52 in Korea
Hyunjoo
Pai,1,*
Sen
Lyu,1
Ji Hyang
Lee,1
Jungmin
Kim,2
Youngmi
Kwon,2
Jong-Won
Kim,3 and
Kang Won
Choe4
Division of Infectious Disease, Department of
Internal Medicine,1 and Department of
Microbiology,2 College of Medicine, University
of Dankook, Chonan, and Department of Clinical Pathology,
Sungkyunkwan University College of
Medicine,3 and Department of Internal
Medicine, Seoul National University College of
Medicine,4 Seoul, Korea
Received 4 November 1998/Returned for modification 16 January
1999/Accepted 24 February 1999
Two hundred ninety isolates of Escherichia coli were
investigated for the production of extended-spectrum
-lactamases
(ESBLs). Fourteen (4.8%) of the 290 strains were found to produce
ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain produced
SHV-12, 1 strain produced a CMY-1-like enzyme, and 1 strain expressed
SHV-2a and a CMY-1-like enzyme. Another two strains for which the MICs
of ceftazidime and cefoxitin were high, were probable AmpC enzyme
hyperproducers. Because of the high prevalence of TEM-52 in E. coli isolates, we further investigated the TEM-type ESBLs
produced by Klebsiella pneumoniae in order to observe the distribution of TEM-52 enzymes among Enterobacteriaceae in
Korea. All TEM enzymes produced by 12 strains of K. pneumoniae were identified as TEM-52. To evaluate the genetic
relatedness among the organisms, ribotyping of TEM-52-producing
E. coli and K. pneumoniae was performed. The
ribotyping profiles of the organisms showed similar but clearly different patterns. In conclusion, TEM-52 is the most prevalent TEM-type ESBL in Korea.
*
Corresponding author. Mailing address: Division of
Infectious Disease, Department of Internal Medicine, College of
Medicine, University of Dankook, San 29, Anseo-dong, Chonan, Chungnam,
330-715, Korea. Phone: 82-417-550-3918. Fax: 82-417-556-3256. E-mail:
paihj{at}unitel.co.kr.
Journal of Clinical Microbiology, June 1999, p. 1758-1763, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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