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Journal of Clinical Microbiology, June 1999, p. 1809-1812, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Typing of Vibrio parahaemolyticus Isolates, Obtained from Patients Involved in Food Poisoning Outbreaks in Taiwan, by Random Amplified Polymorphic DNA Analysis

Hin-Chung Wong,1,* Chi-Chang Liu,1 Tze-Ming Pan,2 Tien-Kuei Wang,2 Chih-Lung Lee,2 and Daniel Yang-Chih Shih3

Department of Microbiology, Soochow University, Taipei, Taiwan 11102,1 and Bacteriology Division, National Institute of Preventive Medicine,2 and Food Microbiology Division, National Laboratories of Foods and Drugs,3 Taipei, Taiwan 11513, Republic of China

Received 23 June 1998/Returned for modification 25 August 1998/Accepted 22 February 1999

Vibrio parahaemolyticus is one of the most important food-borne pathogens in Taiwan, Japan, and other countries with long coastlines. This paper reports on the development of a new random amplified polymorphic DNA (RAPD) method for the molecular typing of this pathogen. The 10-mer primer 284 (5'-CAG GCG CAC A-3') was selected to generate polymorphic amplification profiles of the genomic DNA at an annealing temperature of 38°C. A total of 308 clinical isolates of V. parahaemolyticus collected during food poisoning outbreaks in Taiwan, mostly occurring between 1993 and 1995, plus 11 environmental and clinical reference strains were analyzed by this RAPD method. A total of 41 polymorphic RAPD patterns were recognized, and these patterns were arbitrarily grouped into 16 types (A to P). Types A, B, C, D, and E were the major types, and subtypes C3, C5, E1, B1, D2, and A2 were the major patterns. The major types were phylogenetically more closely related to each other than to any of the minor types.

* Corresponding author. Mailing address: Department of Microbiology, Soochow University, Taipei, Taiwan 1102, Republic of China. Phone: (886) 2-28819471, ext. 6852. Fax: (886) 2-28831193. E-mail: wonghc{at}mail.scu.edu.tw.

Journal of Clinical Microbiology, June 1999, p. 1809-1812, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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